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Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage
http://hdl.handle.net/10061/14622
http://hdl.handle.net/10061/1462281caf45d-60b6-4dfd-b561-271e05de7c82
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-02-15 | |||||
| タイトル | ||||||
| タイトル | Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | preimplantation embryo | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | sex chromosome | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | sexing | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | tdTomato | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirata, Wataru
× Hirata, Wataru× Tomoda, Taiki× Yuri, Shunsuke× Isotani, Ayako |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In mammals, sexual fate is determined by the chromosomes of the male and female gametes during fertilization. Males (XY) or females (XX) are produced when a sperm containing a Y or X-chromosome respectively fertilizes an X-chromosome-containing unfertilized egg. However, sexing of preimplantation stage embryos cannot be conducted visually. To address this, transgenic male mouse models with the ubiquitously expressed green fluorescent protein (GFP) transgene on X- (X-GFP) or Y-chromosomes (Y-GFP) have been established. However, when crossed with wild-type females, sexing of the preimplantation stage embryos by observing the GFP signal is problematic in some cases due to X-inactivation, loss of Y-chromosome (LOY), or loss of transgene fluorescence. In this study, a mouse model with the ubiquitously expressed red fluorescent protein (RFP) transgene on the Y-chromosome was generated since RFP is easily distinguishable from GFP signals. Unfortunately, the ubiquitously expressed tdTomato RFP transgene on the Y-chromosome (Y-RFP) mouse showed the lethal phenotype after birth. No lethal phenotypes were observed when the mitochondrial locating signal N-terminal of tdTomato (mtRFP) was included in the transgene construct. Almost half of the collected fertilized eggs from Y-mtRFP male mice crossed with wild-type females had an RFP signal at the preimplantation stage (E1.5). Therefore, XY eggs were recognized as RFP-positive embryos at the preimplantation stage. Furthermore, 100% sexing was observed at the preimplantation stage using the X-linked GFP/Y-linked RFP male mouse. The established Y-mtRFP mouse models may be used to study sex chromosome related research. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Experimental Animals 巻 71, 号 1, p. 82-89, 発行日 2022-02-09 |
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| 出版者 | ||||||
| 出版者 | Japanese Association for Laboratory Animal Science | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1881-7122 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34544911 | |||||
| 出版者版DOI | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1538/expanim.21-0119 | |||||
| 出版者版URI | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/expanim/71/1/71_21-0119/_pdf/-char/en | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | c2022 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>. | |||||
