{"created":"2023-07-25T10:30:11.032300+00:00","id":9415,"links":{},"metadata":{"_buckets":{"deposit":"30280699-0100-4cc1-b7e6-8d8c0dcff0d7"},"_deposit":{"created_by":4,"id":"9415","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"9415"},"status":"published"},"_oai":{"id":"oai:naist.repo.nii.ac.jp:00009415","sets":["43:44"]},"author_link":["26416","26417","168","68"],"item_7_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"89","bibliographicPageStart":"82","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Experimental Animals","bibliographic_titleLang":"en"}]}]},"item_7_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In mammals, sexual fate is determined by the chromosomes of the male and female gametes during fertilization. Males (XY) or females (XX) are produced when a sperm containing a Y or X-chromosome respectively fertilizes an X-chromosome-containing unfertilized egg. However, sexing of preimplantation stage embryos cannot be conducted visually. To address this, transgenic male mouse models with the ubiquitously expressed green fluorescent protein (GFP) transgene on X- (X-GFP) or Y-chromosomes (Y-GFP) have been established. However, when crossed with wild-type females, sexing of the preimplantation stage embryos by observing the GFP signal is problematic in some cases due to X-inactivation, loss of Y-chromosome (LOY), or loss of transgene fluorescence. In this study, a mouse model with the ubiquitously expressed red fluorescent protein (RFP) transgene on the Y-chromosome was generated since RFP is easily distinguishable from GFP signals. Unfortunately, the ubiquitously expressed tdTomato RFP transgene on the Y-chromosome (Y-RFP) mouse showed the lethal phenotype after birth. No lethal phenotypes were observed when the mitochondrial locating signal N-terminal of tdTomato (mtRFP) was included in the transgene construct. Almost half of the collected fertilized eggs from Y-mtRFP male mice crossed with wild-type females had an RFP signal at the preimplantation stage (E1.5). Therefore, XY eggs were recognized as RFP-positive embryos at the preimplantation stage. Furthermore, 100% sexing was observed at the preimplantation stage using the X-linked GFP/Y-linked RFP male mouse. The established Y-mtRFP mouse models may be used to study sex chromosome related research.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_publisher_10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Association for Laboratory Animal Science","subitem_publisher_language":"en"}]},"item_7_relation_15":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isReplacedBy","subitem_relation_type_id":{"subitem_relation_type_id_text":"34544911","subitem_relation_type_select":"PMID"}}]},"item_7_relation_17":{"attribute_name":"出版者版DOI","attribute_value_mlt":[{"subitem_relation_type":"isReplacedBy","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1538/expanim.21-0119","subitem_relation_type_select":"DOI"}}]},"item_7_relation_22":{"attribute_name":"出版者版URI","attribute_value_mlt":[{"subitem_relation_type":"isReplacedBy","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/expanim/71/1/71_21-0119/_pdf/-char/en","subitem_relation_type_select":"URI"}}]},"item_7_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c2022 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives  (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>.","subitem_rights_language":"en"}]},"item_7_source_id_12":{"attribute_name":"EISSN/PISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-7122","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirata, Wataru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26416","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomoda, Taiki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26417","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yuri, Shunsuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"168","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10800881","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10800881"}]},{"creatorNames":[{"creatorName":"Isotani, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20444523","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20444523"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"preimplantation embryo","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sex chromosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sexing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tdTomato","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage","subitem_title_language":"en"}]},"item_type_id":"7","owner":"4","path":["44"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-02-15"},"publish_date":"2022-02-15","publish_status":"0","recid":"9415","relation_version_is_last":true,"title":["Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-11-29T08:44:14.392524+00:00"}