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Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins
http://hdl.handle.net/10061/13378
http://hdl.handle.net/10061/13378066434af-5472-46b7-8718-79b3aad0c886
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-16 | |||||
タイトル | ||||||
タイトル | Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins | |||||
言語 | ||||||
言語 | eng | |||||
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資源タイプ | journal article | |||||
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アクセス権 | open access | |||||
著者 |
Ichikawa, Muneyoshi
× Ichikawa, Muneyoshi× Khalifa, Ahmad Abdelzaher Zaki× Kubo, Shintaroh× Dai, Daniel× Basu, Kaustuv× Maghrebi, Mohammad Amin Faghfor× Vargas, Javier× Bui, Khanh Huy |
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内容記述タイプ | Abstract | |||||
内容記述 | Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic resolution map of the Tetrahymena doublet microtubule by cryoelectron microscopy. The map demonstrates that the network of microtubule inner proteins weaves into the tubulin lattice and forms an inner sheath. From mass spectrometry data and de novo modeling, we identified Rib43a proteins as the filamentous microtubule inner proteins in the protofilament ribbon region. The Rib43a?tubulin interaction leads to an elongated tubulin dimer distance every 2 dimers. In addition, the tubulin lattice structure with missing microtubule inner proteins (MIPs) by sarkosyl treatment shows significant longitudinal compaction and lateral angle change between protofilaments. These results are evidence that the MIPs directly affect and stabilize the tubulin lattice. It suggests that the doublet microtubule is an intrinsically stressed filament and that this stress could be manipulated in the regulation of ciliary waveforms. | |||||
書誌情報 |
en : Proceedings of the National Academy of Sciences 発行日 2019-09-16 |
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出版者 | National Academy of Sciences | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-8424 | |||||
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関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1073/pnas.1911119116 | |||||
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権利情報 | c 2019 Published under the PNAS license | |||||
権利 | ||||||
権利情報 | 出版社許諾条件により、本文は2020年3月16日以降に公開 | |||||
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出版タイプ | AM |