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In this study, we present a near-atomic resolution map of the Tetrahymena doublet microtubule by cryoelectron microscopy. The map demonstrates that the network of microtubule inner proteins weaves into the tubulin lattice and forms an inner sheath. From mass spectrometry data and de novo modeling, we identified Rib43a proteins as the filamentous microtubule inner proteins in the protofilament ribbon region. The Rib43a?tubulin interaction leads to an elongated tubulin dimer distance every 2 dimers. In addition, the tubulin lattice structure with missing microtubule inner proteins (MIPs) by sarkosyl treatment shows significant longitudinal compaction and lateral angle change between protofilaments. These results are evidence that the MIPs directly affect and stabilize the tubulin lattice. It suggests that the doublet microtubule is an intrinsically stressed filament and that this stress could be manipulated in the regulation of ciliary waveforms.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_publisher_10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Academy of Sciences","subitem_publisher_language":"en"}]},"item_7_relation_17":{"attribute_name":"出版者版DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1073/pnas.1911119116","subitem_relation_type_select":"DOI"}}]},"item_7_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019  Published under the PNAS license","subitem_rights_language":"en"},{"subitem_rights":"出版社許諾条件により、本文は2020年3月16日以降に公開","subitem_rights_language":"ja"}]},"item_7_source_id_12":{"attribute_name":"EISSN/PISSN","attribute_value_mlt":[{"subitem_source_identifier":"0027-8424","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ichikawa, Muneyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"247","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80844662","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80844662"}]},{"creatorNames":[{"creatorName":"Khalifa, Ahmad Abdelzaher Zaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24627","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubo, Shintaroh","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24628","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Dai, Daniel","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24629","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Basu, Kaustuv","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24630","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maghrebi, Mohammad Amin Faghfor","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24631","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vargas, Javier","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bui, Khanh Huy","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24633","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-06"}],"displaytype":"detail","filename":"20190917bs_ichikawa_2.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Press release","objectType":"other","url":"https://naist.repo.nii.ac.jp/record/9237/files/20190917bs_ichikawa_2.pdf"},"version_id":"4e947914-9f58-408b-b105-f0f3c2d3f43a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-06"}],"displaytype":"detail","filename":"2019-11119R_Merged_PDF.pdf","filesize":[{"value":"24.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"fulltext","objectType":"fulltext","url":"https://naist.repo.nii.ac.jp/record/9237/files/2019-11119R_Merged_PDF.pdf"},"version_id":"3b003c4e-194a-4650-ad19-3ca3673ee40f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins","subitem_title_language":"en"}]},"item_type_id":"7","owner":"4","path":["44"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-03-16"},"publish_date":"2020-03-16","publish_status":"0","recid":"9237","relation_version_is_last":true,"title":["Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-11-29T08:50:56.777041+00:00"}