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Carbon monoxide binding properties of domain-swapped dimeric myoglobin
http://hdl.handle.net/10061/10422
http://hdl.handle.net/10061/10422db428451-a7dd-414f-ad49-90a4d900e314
名前 / ファイル | ライセンス | アクション |
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fulltext (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-10-07 | |||||
タイトル | ||||||
タイトル | Carbon monoxide binding properties of domain-swapped dimeric myoglobin | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Myoglobin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Domain swapping | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ligand binding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Active site structure | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
著者 |
Nagao, Satoshi
× Nagao, Satoshi× Ishikawa, Haruto× Yamada, Takuya× Mizutani, Yasuhisa× Hirota, Shun |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Myoglobin (Mb) is a monomeric oxygen storage hemoprotein, and has been shown to form a domain-swapped dimer. In this study, monomeric and dimeric carbon monoxide (CO)-bound Mb (MbCO) exhibited similar absorption spectra. The CO stretching frequencies of MbCO were observed at 1,932 and 1,944 cm-1 for both monomeric and dimeric MbCO. The resonance Raman (RR) bands for the stretching between the heme iron and axial ligands were observed at the same frequencies for the monomer and dimer of deoxygenated Mb (deoxyMb) and MbCO, respectively (ν Fe-His, 220 cm-1; ν Fe-C, 507 cm-1), showing that the Fe-His bond strength of deoxyMb and the Fe-CO bond strength of MbCO did not change by the dimerization. Time-resolved RR measurements showed that the dynamics of the structural changes at the heme active site after CO photo-dissociation of MbCO was similar between monomeric and dimeric Mb [monomer, (5.2 ± 1.8) × 106 s-1; dimer, (6.2 ± 1.1) × 106 s-1 at room temperature]. These results show that the heme coordination structure, the protein environment around the bound CO, and the protein relaxation character are similar between monomeric and dimeric MbCO. Although the active site structure was similar between the monomer and dimer, the CO binding rate constant of dimeric Mb [(1.01 ± 0.03) × 106 M-1 s-1 at 20 °C] was about twice larger than that of the monomer [(0.52 ± 0.02) × 106 M-1 s-1 at 20 °C], presumably due to the expansion of the channel between the Xe3 cavity and the solvent by the dimerization. | |||||
書誌情報 |
en : JBIC Journal of Biological Inorganic Chemistry 巻 20, 号 3, p. 523-530, 発行日 2015-04-01 |
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出版者 | ||||||
出版者 | Springer Berlin Heidelberg | |||||
EISSN/PISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0949-8257 | |||||
出版者版DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00775-014-1236-0 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11133001 | |||||
権利 | ||||||
権利情報 | 出版社許諾条件により、本文は2016年1月13日以降公開 | |||||
権利 | ||||||
権利情報 | c Springer International Publishing AG, Part of Springer Science+Business Media | |||||
権利 | ||||||
権利情報 | The final publication is available at Springer via http://dx.doi.org/10.1007/s00775-014-1236-0 | |||||
著者版フラグ | ||||||
出版タイプ | AM |