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High-level production of ornithine by expression of the feedback inhibition-insensitive N-acetyl glutamate kinase in the sake yeast Saccharomyces cerevisiae
http://hdl.handle.net/10061/14447
http://hdl.handle.net/10061/1444729e7b454-6a5b-49fe-933b-4eac8468f302
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-09-10 | |||||
タイトル | ||||||
タイトル | High-level production of ornithine by expression of the feedback inhibition-insensitive N-acetyl glutamate kinase in the sake yeast Saccharomyces cerevisiae | |||||
その他のタイトル | ||||||
その他のタイトル | 清酒酵母におけるフィードバック阻害非感受性型N-アセチルグルタミン酸キナーゼの発現によるオルニチン高生産 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Saccharomyces cerevisiae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sake yeast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ornithine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | N-acetyl glutamate kinase Arg5,6 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Feedback inhibition | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
著者 |
Ohashi, Masataka
× Ohashi, Masataka× Nasuno, Ryo× Isogai, Shota× Takagi, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We previously reported that intracellular proline (Pro) confers tolerance to ethanol on the yeast Saccharomyces cerevisiae. In this study, to improve the ethanol productivity of sake, a traditional Japanese alcoholic beverage, we successfully isolated several Pro-accumulating mutants derived from diploid sake yeast of S. cerevisiae by a conventional mutagenesis. Interestingly, one of them (strain A902-4) produced more than 10-fold greater amounts of ornithine (Orn) and Pro compared to the parent strain (K901). Orn is a non-proteinogenic amino acid and a precursor of both arginine (Arg) and Pro. It has some physiological functions, such as amelioration of negative states such as lassitude and improvement of sleep quality. We also identified a homo-allelic mutation in the ARG5,6 gene encoding the Thr340Ile variant N-acetylglutamate kinase (NAGK) in strain A902-4. The NAGK activity of the Thr340Ile variant was extremely insensitive to feedback inhibition by Arg, leading to intracellular Orn accumulation. This is the first report of the removal of feedback inhibition of NAGK activity in the industrial yeast, leading to high levels of intracellular Orn. Moreover, sake and sake cake brewed with strain A902-4 contained 4?5 times more Orn than those brewed with strain K901. The approach described here could be a practical method for the development of industrial yeast strains with overproduction of Orn. | |||||
書誌情報 |
en : Metabolic Engineering 巻 62, p. 1-9, 発行日 2020-11-01 |
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出版者 | ||||||
出版者 | Elsevier | |||||
出版者URL | ||||||
権利情報 | https://www.sciencedirect.com/science/article/pii/S1096717620301257 | |||||
EISSN/PISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1096-7176 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 32805427 | |||||
出版者版DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.ymben.2020.08.005 | |||||
権利 | ||||||
権利情報 | c 2020 The Authors. Published by Elsevier Inc. on behalf of International Metabolic Engineering Society. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR |