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Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse
http://hdl.handle.net/10061/14029
http://hdl.handle.net/10061/140296c709084-6ce8-42fa-8db4-3acf51bdb9d3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-25 | |||||
| タイトル | ||||||
| タイトル | Hereditary Pancreatitis Model by Blastocyst Complementation in Mouse | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | blastocyst complementation; | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | hereditary pancreatitis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | hereditary pancreatitis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | PRSS1 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Asai, Ayumu
× Asai, Ayumu× Konno, Masamitsu× Kawamoto, Koichi× Isotani, Ayako× Mori, Masaki× Eguchi, Hidetoshi× Doki, Yuichiro× Arai, Takahiro× Ishii, Hideshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The application of pluripotent stem cells is expected to contribute to the elucidation of unknown mechanism of human diseases. However, in vitro induction of organ-specific cells, such as pancreas and liver, is still difficult and the reproduction of their disorders in a model has been unfeasible. To study the mechanism of human hereditary pancreatitis (HP), we here performed the blastocyst complementation (BC) method. In the BC method, mouse embryonic stem (ES) cells harboring CRISPR/CAS9-mediated mutations in the Prss1 gene were injected into blastocysts with deficient Pdx1 gene, which is a critical transcription factor in the development of pancreas. The results showed that trypsin was activated extremely in Prss1-mutant mice. This implied that the mouse phenotype mimics that of human HP and that the BC method was useful for the reproduction and study of pancreatic disorders. The present study opens the possibility of investigating uncharacterized human diseases by utilizing the BC method. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Oncotarget 巻 11, 号 22, p. 2061-2073, 発行日 2020-06-02 |
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| 出版者 | ||||||
| 出版者 | Impact Journals | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1949-2553 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32547704 | |||||
| 出版者版DOI | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.18632/oncotarget.27595 | |||||
| 出版者版URI | ||||||
| 関連タイプ | isReplacedBy | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.oncotarget.com/article/27595/text/ | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Asai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and sourceare credited. | |||||
