WEKO3
アイテム
A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis
http://hdl.handle.net/10061/12589
http://hdl.handle.net/10061/125897fc70606-a4f1-4abf-bc20-0601ab5a8c4a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fulltext (28.0 MB)
|
|
|
Movie S1.avi (4.6 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-10-05 | |||||
| タイトル | ||||||
| タイトル | A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
Yin, Ke
× Yin, Ke× Ueda, Minako× Takagi, Hitomi× Kajihara, Takehiro× Sugamata, Aki Shiori× Nobusawa, Takashi× Umeda-Hara, Chikage× Umeda, Masaaki |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Visualization of the spatiotemporal pattern of cell division is crucial to understand how multicellular organisms develop and how they modify their growth in response to varying environmental conditions. The mitotic cell cycle consists of four phases: S (DNA replication), M (mitosis and cytokinesis), and the intervening G1 and G2 phases; however, only G2/M‐specific markers are currently available in plants, making it difficult to measure cell cycle duration and to analyze changes in cell cycle progression in living tissues. Here, we developed another cell cycle marker that labels S‐phase cells by manipulating Arabidopsis CDT1a, which functions in DNA replication origin licensing. Truncations of the CDT1a coding sequence revealed that its carboxy‐terminal region is responsible for proteasome‐mediated degradation at late G2 or in early mitosis. We therefore expressed this region as a red fluorescent protein fusion protein under the S‐specific promoter of a histone 3.1‐type gene, HISTONE THREE RELATED2 (HTR2), to generate an S/G2 marker. Combining this marker with the G2/M‐specific CYCB1‐GFP marker enabled us to visualize both S to G2 and G2 to M cell cycle stages, and thus yielded an essential tool for time‐lapse imaging of cell cycle progression. The resultant dual‐color marker system, Cell Cycle Tracking in Plant Cells (Cytrap), also allowed us to identify root cells in the last mitotic cell cycle before they entered the endocycle. Our results demonstrate that Cytrap is a powerful tool for in vivo monitoring of the plant cell cycle, and thus for deepening our understanding of cell cycle regulation in particular cell types during organ development. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 動画は環境により再生できない場合ありmac OS High Sierra バージョン 10.13.1 動作確認済 | |||||
| 言語 | ja | |||||
| 書誌情報 |
en : The Plant Journal 巻 80, 号 3, p. 541-552, 発行日 2014-08-27 |
|||||
| 出版者 | ||||||
| 出版者 | Wiley/Blackwell (10.1111) | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0960-7412 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 25158977 | |||||
| 出版者版DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/tpj.12652 | |||||
| 収録物識別子 | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10846815 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | c 2014 The Authors The Plant Journal c 2014 John Wiley & Sons Ltd | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This is the peer reviewed version of the following article: [The Plant Journal, 80(3):541-552], which has been published in final form at [https://doi.org/10.1111/tpj.12652]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
