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Identification and characterization of a second, inducible promoter of relA in Escherichia coli
http://hdl.handle.net/10061/6660
http://hdl.handle.net/10061/66603cc1c1a8-3c20-441d-9216-ac0ab0ada2d4
名前 / ファイル | ライセンス | アクション |
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fulltext (683.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-02-01 | |||||
タイトル | ||||||
タイトル | Identification and characterization of a second, inducible promoter of relA in Escherichia coli | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
著者 |
Nakagawa, Akira
× Nakagawa, Akira× Oshima, Taku× Mori, Hirotada |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The alarmone ppGpp is an important signal molecule for the stringent response. Escherichia coli relA encodes a ppGpp synthetase, and although the regulation of RelA protein activity has been studied extensively, the regulation of relA transcription remains unclear. Here, we describe a novel relA promoter, relAP2. According to quantitative measurement of mRNA by primer extension analysis, the previously reported promoter relAP1 is constitutively active throughout growth, while relAP2 is induced temporarily at the transition state between the exponential growth and stationary phases. A chromosomal transcriptional lacZ fusion (relAP2-lacZ) showed that relAP2 is positively regulated by H-NS and CRP. Furthermore, the reduced activity of relAP2-lacZ in an hns mutant could be rescued by an rpoS mutation, which is sufficient to derepress the relAP2-lacZ activity. These data suggest that transient expression from the relAP2 promoter is controlled by several global regulators. This may account for the complex regulation of relA expression in Escherichia coli. | |||||
書誌情報 |
en : GENES & GENETIC SYSTEMS 巻 81, 号 5, p. 299-310, 発行日 2006-10 |
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出版者 | ||||||
出版者 | Genetics Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0024-4589 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1266/ggs.81.299 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10584071 | |||||
権利 | ||||||
権利情報 | Copyright (C) 2006 Genetics Society of Japan . | |||||
著者版フラグ | ||||||
出版タイプ | VoR |