{"created":"2023-07-25T10:30:24.230629+00:00","id":9685,"links":{},"metadata":{"_buckets":{"deposit":"5232da22-cbc6-4ba0-9bad-c906e22ba92a"},"_deposit":{"created_by":4,"id":"9685","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"9685"},"status":"published"},"_oai":{"id":"oai:naist.repo.nii.ac.jp:00009685","sets":["49:50"]},"author_link":["27326","27327","27328","144","27329","27330","4"],"item_7_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"288","bibliographicPageStart":"284","bibliographicVolumeNumber":"430","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_7_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[NiFe] hydrogenase catalyzes reversible oxidation of molecular hydrogen. Its active site is constructed of a hetero dinuclear Ni-Fe complex, and the oxidation state of the Ni ion changes according to the redox state of the enzyme. We found that the Ni-A state (an inactive unready, oxidized state) of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F (DvMF) is light sensitive and forms a new state (Ni-AL) with irradiation of visible light. The Fourier transform infrared (FT-IR) bands at 1956, 2084 and 2094 cm^-1 of the Ni-A state shifted to 1971, 2086 and 2098 cm^-1 in the Ni-AL state. The g-values of gx = 2.30, gy = 2.23 and gz = 2.01 for the signals in the electron paramagnetic resonance (EPR) spectrum of the Ni-A state at room temperature varied for -0.009, +0.012 and +0.010, respectively, upon light irradiation. The light-induced Ni-AL state converted back immediately to the Ni-A state under dark condition at room temperature. These results show that the coordination structure of the Fe site of the Ni-A state of [NiFe] hydrogenase is perturbed significantly by light irradiation with relatively small coordination change at the Ni site.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_publisher_10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_7_relation_17":{"attribute_name":"出版者版DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2012.10.136","subitem_relation_type_select":"DOI"}}]},"item_7_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2012 Elsevier Inc. All rights reserved.","subitem_rights_language":"en"}]},"item_7_source_id_12":{"attribute_name":"EISSN/PISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_14":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_7_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Osuka, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shomura, Yasuhito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komori, Hirofumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27328","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibata, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40335477","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40335477"}]},{"creatorNames":[{"creatorName":"Nagao, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27329","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Yoshiki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27330","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirota, Shun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90283457","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90283457"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-06"}],"displaytype":"detail","filename":"BBRC2013.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"fulltext","objectType":"fulltext","url":"https://naist.repo.nii.ac.jp/record/9685/files/BBRC2013.pdf"},"version_id":"6feddfc1-b7d1-40c3-be2e-270aeb878923"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"[NiFe] hydrogenase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Desulfovibrio vulgaris Miyazaki F","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ni-A state","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Light sensitivity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FT-IR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EPR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Photosensitivity of the Ni-A state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F with visible light","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Photosensitivity of the Ni-A state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F with visible light","subitem_title_language":"en"}]},"item_type_id":"7","owner":"4","path":["50"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-09-25"},"publish_date":"2015-09-25","publish_status":"0","recid":"9685","relation_version_is_last":true,"title":["Photosensitivity of the Ni-A state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F with visible light"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-11-29T09:06:30.082268+00:00"}