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Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector
http://hdl.handle.net/10061/13986
http://hdl.handle.net/10061/13986d31f93e2-99a6-4ff1-8fc7-5a4d40dcccdc
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HPLCNO Anal Biochem Revised Figures.pdf (499.1 kB)
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HPLCNO Anal Biochem Revised Text.pdf (167.4 kB)
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HPLCNO Anal Biochem Table.pdf (41.9 kB)
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fulltext (1.2 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-05-28 | |||||
| タイトル | ||||||
| タイトル | Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nitric oxide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fluorescence probe | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Methodology | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Yeast | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Environmental stress | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 著者 |
Nasuno, Ryo
× Nasuno, Ryo× Shino, Seiya× Yoshikawa, Yuki× Yoshioka, Natsuko× Sato, Yuichi× Kamiya, Kohei× Hiroshi, Takagi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nitric oxide (NO) is an important signaling molecule involved in various biological phenomena in many organisms. The physiological functions and metabolism of NO in yeast, a unicellular microorganism, are still unknown, mainly because it is difficult to analyze the intracellular NO levels accurately. Here, we developed a new method of more accurately measuring NO content in yeast cells with the detection limit of 6 nM, by treating the cells with an NO-specific fluorescence probe followed by high-performance liquid chromatography with fluorescence detection (HPLC/FLD). This approach successfully detected and quantified the NO content inside yeast cells treated with an NO donor. Moreover, the HPLC/FLD analysis indicates that the fluorescence induced under some environmental stress conditions, such as ethanol, vanillin, and heat-shock, was not derived from NO. The HPLC/FLD method developed in this study provides a new strategy for measuring the intracellular NO concentration with higher accuracy. | |||||
| 書誌情報 |
en : Analytical Biochemistry 巻 598, 発行日 2020-06-01 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0003-2697 | |||||
| 出版者版DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.ab.2020.113707 | |||||
| 権利 | ||||||
| 権利情報 | c 2020 Elsevier Inc. All rights reserved. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
