@article{oai:naist.repo.nii.ac.jp:00009151,
 author = {Yamamoto, Natsuko and Nakahigashi, Kenji and Nakamichi, Tomoko and Yoshino, Mihoko and Takai, Yuki and Touda, Yae and Furubayashi, Akemi and Kinjyo, Satoko and Dose, Hitomi and Hasegawa, Miki and Datsenko, Kirill A and Nakayashiki, Toru and Tomita, Masaru and Wanner, Barry L and Mori, Hirotada},
 issue = {1},
 journal = {Molecular Systems Biology},
 month = {Dec},
 note = {The Keio collection (Baba et al, 2006) has been established as a set of single‐gene deletion mutants of Escherichia coli K‐12. These mutants have a precisely designed deletion from the second codon from the seventh to the last codon of each predicted ORF. Further information is available at http://sal.cs.purdue.edu:8097/GB7/index.jsp or http://ecoli.naist.jp/. The distribution is now being handled by the National Institute of Genetics of Japan (http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp). To date more than 4 million samples have been distributed worldwide. As we described earlier (Baba et al, 2006), gene amplification during construction is likely to have led to a small number of mutants with genetic duplications.},
 title = {Update on the Keio collection of Escherichia coli single‐gene deletion mutants},
 volume = {5},
 year = {2009}
}