{"created":"2023-07-25T10:29:57.506493+00:00","id":9126,"links":{},"metadata":{"_buckets":{"deposit":"7a75a5e9-97ad-4ee7-86ee-1620efd249f6"},"_deposit":{"created_by":4,"id":"9126","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"9126"},"status":"published"},"_oai":{"id":"oai:naist.repo.nii.ac.jp:00009126","sets":["43:44"]},"author_link":["23418","23419","23420","339","23421","23422"],"item_7_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02-29","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Plant & Cell Physiology","bibliographic_titleLang":"en"}]}]},"item_7_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments and cytoplasmic components. The techniques allow fluorescence-based detailed analyses of materials and characterization of the three-dimensional structure of organs. We describe a simple and rapid clearing and imaging method, termed ‘TOMEI’ (Transparent plant Organ MEthod for Imaging), which enables microscopic observation of intact plant organs. This method involves a clearing reagent containing 2,2′-thiodiethanol. Conveniently, transparent plant organs were prepared within only 3-6 h. We detected fluorescent stains at a depth of approximately 200 μm using confocal laser scanning microscopy and analyzed fluorescent proteins in internal tissues of transparent organs cleared using TOMEI. We adapted TOMEI for various plant organs of Arabidopsis thaliana and Oryza sativa, including leaves, flower buds, flower stalks, root and nematode-infected root-knots. We visualized whole leaves of A. thaliana from the adaxial epidermis to the abaxial epidermis as well as protoxylem and metaxylem vessels of vascular bundles embedded in spongy mesophyll cells. Inner floral organs were observed in flower buds cleared using TOMEI without the need to prepare sections or remove sepals. Multicolor imaging of fluorescent proteins and dyes, and analyses of the three-dimensional structure of plant organs based on optical sections are possible using TOMEI. We analyzed root-knots cleared using TOMEI and revealed that nematodes induce giant cell expansion in a DNA content-dependent manner. The TOMEI method is applicable to analysis of fluorescent proteins and dyes quantitatively with cell morphological characteristics in whole plant organs.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_publisher_10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press","subitem_publisher_language":"en"}]},"item_7_relation_17":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/pcp/pcw027","subitem_relation_type_select":"DOI"}}]},"item_7_relation_22":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://pcp.oxfordjournals.org/content/early/2016/02/29/pcp.pcw027","subitem_relation_type_select":"URI"}}]},"item_7_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"出版社許諾条件により、本文は2017年3月1日以降公開 The publisher require to delay the availability of full text until 2017/3/1.","subitem_rights_language":"ja"},{"subitem_rights":"This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant & Cell Phsyology following peer review. The version of record Plant Cell Physiol First published online: February 29, 2016 is available online at: http://pcp.oxfordjournals.org/content/early/2016/02/29/pcp.pcw027.","subitem_rights_language":"en"}]},"item_7_source_id_12":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1471-9053","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Junko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23418","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakamoto, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23419","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakagami, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23420","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aida, Mitsuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"339","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90311787","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90311787"}]},{"creatorNames":[{"creatorName":"Sawa, Shinichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23421","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsunaga, Sachihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23422","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-06"}],"displaytype":"detail","filename":"2016_PCP.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"fulltext","objectType":"fulltext","url":"https://naist.repo.nii.ac.jp/record/9126/files/2016_PCP.pdf"},"version_id":"a21e6e2d-4b99-4977-83c1-0ca4dc0660eb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"2,2′-Thiodiethanol","subitem_subject_scheme":"Other"},{"subitem_subject":"Clearing method","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Deep imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescent protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transparency technique","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique","subitem_title_language":"en"}]},"item_type_id":"7","owner":"4","path":["44"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-03-01"},"publish_date":"2017-03-01","publish_status":"0","recid":"9126","relation_version_is_last":true,"title":["Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-11-21T07:31:49.972654+00:00"}