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Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism
http://hdl.handle.net/10061/8821
http://hdl.handle.net/10061/882135eb502d-bc7b-49ce-943d-a8e8fb6779d1
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-07-05 | |||||
| タイトル | ||||||
| タイトル | Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cyclic amine N-acetyltransferase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | X-ray crystallography | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | reaction mechanism | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | antioxidant enzyme | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 著者 |
Nasuno, Ryo
× Nasuno, Ryo× Hirano, Yoshinori× Itoh, Takafumi× Hakoshima, Toshio× Hibi, Takao× Takagi, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mpr1 (sigma1278b gene for proline-analog resistance 1), which was originally isolated as N-acetyltransferase detoxifying the proline analog L-azetidine-2-carboxylate, protects yeast cells from various oxidative stresses. Mpr1 mediates the L-proline and L-arginine metabolism by acetylating L-Δ1-pyrroline-5-carboxylate, leading to the L-arginine-dependent production of nitric oxide, which confers oxidative stress tolerance. Mpr1 belongs to the Gcn5-related N-acetyltransferase (GNAT) superfamily, but exhibits poor sequence homology with the GNAT enzymes and unique substrate specificity. Here, we present the X-ray crystal structure of Mpr1 and its complex with the substrate cis-4-hydroxy-L-proline at 1.9 and 2.3 A resolution, respectively. Mpr1 is folded into α/β-structure with eight-stranded mixed β-sheets and six α-helices. The substrate binds to Asn135 and the backbone amide of Asn172 and Leu173, and the predicted acetyl-CoA-binding site is located near the backbone amide of Phe138 and the side chain of Asn178. Alanine substitution of Asn178, which can interact with the sulfur of acetyl-CoA, caused a large reduction in the apparent kcat value. The replacement of Asn135 led to a remarkable increase in the apparent Km value. These results indicate that Asn178 and Asn135 play an important role in catalysis and substrate recognition, respectively. Such a catalytic mechanism has not been reported in the GNAT proteins. Importantly, the amino acid substitutions in these residues increased the L-Δ1-pyrroline-5-carboxylate level in yeast cells exposed to heat stress, indicating that these residues are also crucial for its physiological functions. These studies provide some benefits of Mpr1 applications, such as the breeding of industrial yeasts and the development of antifungal drugs. | |||||
| 書誌情報 |
en : Proceedings of the National Academy of Sciences of the United States of America 巻 110, 号 29, p. 11821-11826, 発行日 2013-07-01 |
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| 出版者 | ||||||
| 出版者 | National Academy of Sciences | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1091-6490 | |||||
| 出版者版DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1073/pnas.1300558110 | |||||
| 収録物識別子 | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11726874 | |||||
| 権利 | ||||||
| 権利情報 | Copyright c 2013 National Academy of Sciences | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
