| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-31 |
| タイトル |
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タイトル |
The Knock-In Atlas: A web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
花井, 悠真
Hilario, Patrick Louis Lagman
Shiraishi, Yuriko
Yoshida, Nobuyasu
Murakami, Suzuna
Shimizu, Yuji
加納, 規資
Kojima, Minami
Murai, Kokoro
河合, 太郎
岡村, 勝友
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Various cell engineering techniques have been developed by leveraging the CRISPR-Cas9 technology, but large-scale resources for targeted gene knock-in are still limited. Here we introduce the Knock-in Atlas, a web resource for gene tagging by fluorescent proteins by inserting artificial exons in target gene introns. To produce knock-in cells efficiently and reproducibly, we carefully chose and catalogued guide RNAs (gRNAs) for targeting genes in the human and mouse genomes by taking the gRNA efficacy scores and protein structures around the insertion sites into account. As of August 2025, we have characterized knock-in cell lines for 350 proteins, with a focus on RNA binding proteins, by flow cytometry and confocal microscopy. The transfection and flow cytometry protocols were optimized for several cell lines including HEK293T, eHAP1, HeLa, THP-1, Neuro2a, mouse embryonic fibroblast (MEF) and mouse embryonic stem cell (mESC). A website has been launched to organize the results of initial characterization including flow cytometry data after transfection, confocal microscopy, and western blot results for the genes for which knock-in HEK293T cell lines were already made. The site also provides a database to organize the information of pre-designed gRNAs for the human and mouse genomes. <https://rnabio.naist.jp/atlas/>. |
| 書誌情報 |
en : Nucleic Acids Research
巻 53,
号 19,
p. 1-14,
ページ数 14,
発行日 2025-10-21
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1362-4962 |
| 出版者版DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/nar/gkaf1050 |
| 出版者版URI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
URI |
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関連識別子 |
https://academic.oup.com/nar/article/53/19/gkaf1050/8294361 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by-nc/4.0/ |
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権利情報 |
© The Author(s) 2025. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. |
| 著者版フラグ |
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出版タイプ |
VoR |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
24KJ1692 |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24KJ1692/ |
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研究課題名 |
CRISPRノックインスクリーニングによる凝集化しやすい細胞質液滴組成の同定 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
25KJ1828 |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25KJ1828/ |
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研究課題名 |
DroshaアイソフォームによるES/生殖細胞特異的マイクロRNA調節機構の解明 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
17K20145 |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K20145/ |
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研究課題名 |
ｍｉＲＮＡ生合成の調節機構とその影響のゲノムワイド解析 |
| 助成情報 |
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助成機関名 |
Takeda Science Foundation |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
20H03468 |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20H03468/ |
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研究課題名 |
自然免疫受容体を介するPAMPs/DAMPs認識機構と炎症疾患への関与について |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
23K14546 |
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23K14546/ |
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研究課題名 |
エンハンサーRNAの発現制御を介した炎症応答制御機構 |
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