| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-02-27 |
| タイトル |
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タイトル |
Proteome Analysis of the Specific Proteins From Japanese Birch Callus Infected With a Canker-Rot Fungus Inonotus Obliquus Strain IO-U1 (NBRC 113406) |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
infection-specific protein |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Inonotus obliquus |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Japanese birch |
| キーワード |
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主題Scheme |
Other |
|
主題 |
proteome analysis |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Agnestisia, Retno
Chino, Rei
Nezu, Ikumi
Ishiguri, Futoshi
峠, 隆之
Yokota, Shinso
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The specific proteins from Japanese birch (Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker-rot fungus Inonotus obliquus strain IO-U1 (NBRC 113406) at the early infection stage were identified using proteome analysis. The intact, wounded, and infected calli were prepared, and the protein samples obtained at 2 days post-treatments were subjected to two-dimensional electrophoresis (2-DE) for detecting infection-specific proteins. The infection-specific proteins were then analysed using LC/MS/MS, and the obtained data were subjected to the NCBIprot database for identifying the proteins based on homologous sequences search. A total of 134 protein spots on the 2-DE gel were detected as infection-specific proteins. Of these spots, 10 highly expressed proteins were identified as protochlorophyllide reductase chloroplastic (spot ID 1564), enolase (spot ID 368), mitochondrial-processing peptidase subunit alpha-like (spot ID 441), mitochondrial-processing peptidase subunit alpha isoform B (spot ID 1578), T-complex protein 1 subunit eta (spot ID 356), glutathione S-transferase-like protein (spot ID 1121), glutathione S-transferase-like protein, partial (spot ID 1527), protein WALLS ARE THIN 1-like (spot ID 1444), and 1 Sc-3 (spot IDs 1290 and 1322). The obtained results suggest that Japanese birch callus is capable of inducing several proteins related to photosynthesis, energy production, protein import machinery in mitochondria, protein folding, detoxification, secondary cell wall formation, and PR-10 protein at the early infection stage as responses to defend itself against I. obliquus strain IO-U1 infection. |
| 書誌情報 |
en : Forest Pathology
巻 55,
号 6,
p. 1-12,
ページ数 12,
発行日 2025-12-11
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1439-0329 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/efp.70051 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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|
関連識別子 |
https://onlinelibrary.wiley.com/doi/full/10.1111/efp.70051 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2025 The Author(s). This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in anymedium, provided the original work is properly cited, the use is non- commercial and no modifications or adaptations are made. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
17K07833 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07833/ |
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研究課題名 |
癌腫病菌に感染したシラカンバ植物体に生成するタンパク質の3次元画像網羅解析 |
