| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-01-15 |
| タイトル |
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|
タイトル |
Repeated emergence of giant microRNA hairpins across invertebrates |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
microRNA hairpin |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Drosha |
| キーワード |
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主題Scheme |
Other |
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主題 |
Microprocessor |
| キーワード |
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主題Scheme |
Other |
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主題 |
Dicer |
| キーワード |
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主題Scheme |
Other |
|
主題 |
small RNA |
| キーワード |
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主題Scheme |
Other |
|
主題 |
metazoan evolution |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Javad-zada, Mir-Mammad
Zou, Xiaodong
岡村, 勝友
Shang, Renfu
Garg, Ankur
Lee, Ethan
Joshua-Tor, Leemor
Fromm, Bastian
Lai, Eric C.
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
While canonical microRNA (miRNA) hairpins typically bear 30–35 base pair (bp) stems and <15 nucleotide terminal loops, certain miRNA hairpins are much longer. While vertebrates lack long miRNA hairpins, these emerged multiple times within invertebrate lineages. Systematic assessments across >1,400 genomes provided evolutionary insights into the elongation of well-conserved miRNA precursors, which can harbor >1 kb between conserved miRNA and star species and generally form highly base-paired structures. Experiments in Drosophila and humans showed that flies were preferentially capable of maturing certain long precursors, but human cells had a partial capacity. However, neither could handle extreme miRNA hairpins. Finally, analysis of structural variants revealed that extensive stem structure and a local bulge near the dicing site are critical for biogenesis of a lengthened miRNA precursor; the latter likely represents an internal DGCR8 interaction platform. Altogether, we document unanticipated structural complexity in conserved miRNAs and emphasize bioinformatic challenges for their complete annotation. |
| 書誌情報 |
en : Cell Reports
巻 44,
号 9,
p. 1-22,
ページ数 22,
発行日 2025-09-10
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| 出版者 |
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出版者 |
Cell Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2211-1247 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.celrep.2025.116243 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.sciencedirect.com/science/article/pii/S2211124725010149 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2025 The Authors. Published by Elsevier Inc.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
17K20145 |
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|
研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K20145/ |
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研究課題名 |
ｍｉＲＮＡ生合成の調節機構とその影響のゲノムワイド解析 |
| 助成情報 |
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助成機関名 |
Ohsumi Frontier Science Foundation |
| 助成情報 |
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助成機関名 |
Tromsø Research Foundation |
| 助成情報 |
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助成機関名 |
Howard Hughes Medical Institute |
| 助成情報 |
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助成機関名 |
National Institutes of Health (NIH) |
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研究課題番号 |
R01-GM083300 |
| 助成情報 |
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助成機関名 |
National Institutes of Health (NIH) |
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研究課題番号 |
R01-HD108914 |
| 助成情報 |
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|
助成機関名 |
Memorial Sloan Kettering Cancer Center (MSK) |
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研究課題番号 |
P30-CA008748 |
