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Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis
http://hdl.handle.net/10061/0002001298
http://hdl.handle.net/10061/0002001298e19db189-18c5-4b1b-886d-50ab04355567
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2025-12-18 | |||||||||||||||
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| タイトル | Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| 著者 |
Pettkó-Szandtner, Aladár
× Pettkó-Szandtner, Aladár
× Magyar, Zoltan
× 小牧, 伸一郎
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | The kinetochore, critical for accurate chromosome segregation and genome stability in eukaryotes, comprises the Constitutive Centromere Associated Network (CCAN) and the KMN network. In animals, the CCAN associates with centromeric nucleosomes throughout the cell cycle, while the KMN network assembles at kinetochores during M phase, binding spindle microtubules and serving as a platform for the spindle assembly checkpoint (SAC) complex. Despite conserved functions, kinetochore components vary across organisms. In this study, we investigated the subcellular localization and interaction maps of core kinetochore components in Arabidopsis (Arabidopsis thaliana). Of the four conserved CCAN components, we found that only Centromere protein C (CENP-C) localizes to kinetochores, while all KMN components consistently localize to the kinetochore throughout the cell cycle. Immunoprecipitation assays revealed interactions between core kinetochore proteins and regulators involved in DNA replication, histone modification, and chromatin remodeling, suggesting that the kinetochore may also function outside of M phase. Examining interactions between kinetochore and SAC components allowed us to elucidate plant-specific SAC localization mechanisms, providing a functional framework for understanding plant kinetochores and offering insights into SAC regulation in plants. | |||||||||||||||
| 書誌情報 |
en : Plant Physiology 巻 199, 号 2, p. 1-14, ページ数 14, 発行日 2025-09-30 |
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| 出版者 | Oxford University Press | |||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 1532-2548 | |||||||||||||||
| 出版者版DOI | ||||||||||||||||
| 関連タイプ | isReplacedBy | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.1093/plphys/kiaf461 | |||||||||||||||
| 出版者版URI | ||||||||||||||||
| 関連タイプ | isReplacedBy | |||||||||||||||
| 識別子タイプ | URI | |||||||||||||||
| 関連識別子 | https://academic.oup.com/plphys/article/199/2/kiaf461/8268165 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||||||||||||
| 権利情報 | © The Author(s) 2025. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | NA | |||||||||||||||
| 助成情報 | ||||||||||||||||
| 助成機関名 | Arabidopsis Biological Resource Center (ABRC) | |||||||||||||||
| 助成情報 | ||||||||||||||||
| 助成機関名 | NAIST Life Science Collaboration Center (LiSCo) | |||||||||||||||
| 助成情報 | ||||||||||||||||
| 助成機関名 | Japan Society for the Promotion of Science (JSPS) | |||||||||||||||
| 研究課題番号 | 24K09503 | |||||||||||||||
| 研究課題番号URI | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K09503/ | |||||||||||||||
| 研究課題名 | キネトコア構造から探る植物ゲノムの可塑性 | |||||||||||||||
| 助成情報 | ||||||||||||||||
| 助成機関名 | NKFI | |||||||||||||||
| 研究課題番号 | NKFI-139202 | |||||||||||||||
| 研究課題名 | Hungarian Research Funding | |||||||||||||||
