| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-08 |
| 日付 |
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日付 |
2026-04-01 |
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日付タイプ |
Available |
| タイトル |
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タイトル |
Artificial induction of the UPR by Tet-off system-dependent expression of Hac1 and its application in Saccharomyces cerevisiae cells |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
UPR |
| キーワード |
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主題Scheme |
Other |
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主題 |
Hac1 |
| キーワード |
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主題Scheme |
Other |
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主題 |
endoplasmic reticulum |
| キーワード |
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主題Scheme |
Other |
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主題 |
yeast |
| キーワード |
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主題Scheme |
Other |
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主題 |
lipid biosynthesis |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
embargoed access |
| 著者 |
木俣, 有紀
Monguchi, Masaki
Geronimo, Ralph Allen Capistrano
Sugimoto, Maya
木俣, 行雄
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In response to endoplasmic reticulum (ER) stress, yeast Saccharomyces cerevisiae cells produce Hac1, which is a transcription factor responsible for the unfolded protein response (UPR). When Hac1 is unregulatedly expressed from a constitutive promoter, the ER is artificially enforced and enlarged, even without ER stress stimuli. However, such cells are unsuitable for applicative bioproduction because they grow quite slowly and quickly lose their high-UPR phenotype upon their long-term storage. To avoid this problem, we constructed S. cerevisiae plasmids for Hac1 expression under the control of the inducible Tet-off promoter. Yeast cells carrying these plasmids did not exhibit a considerable UPR and grew rapidly when the Tet-off promoter was repressed by doxycycline. In contrast, under the Tet-off inducing condition, these plasmids caused UPR induction, growth retardation, and ER expansion, depending on the copy number of the plasmid. Moreover, as expected, lipidic molecule production was increased under these conditions. |
| 書誌情報 |
en : Bioscience, Biotechnology & Biochemistry
巻 89,
号 4,
p. 562-572,
ページ数 11,
発行日 2025-04-01
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1347-6947 |
| 出版者版DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/bbb/zbaf006 |
| 出版者版URI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
URI |
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関連識別子 |
https://academic.oup.com/bbb/article-abstract/89/4/562/8015780 |
| 権利 |
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権利情報 |
© The Author(s) 2025. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry. This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience, Biotechnology & Biochemistry following peer review. The version of record Ishiwata-Kimata Yuki, Monguchi Masaki, Geronimo Ralph Allen Capistrano, Sugimoto Maya, Kimata Yukio. 2025. Artificial induction of the UPR by Tet-off system-dependent expression of Hac1 and its application in Saccharomyces cerevisiae cells. Bioscience, Biotechnology, and Biochemistry. Volume 89, Issue 4, April 2025, Pages 562-572. is available online at: https://doi.org/10.1093/bbb/zbaf006 . 出版社許諾条件により、本文は2026年4月1日以降に公開。 |
| 著者版フラグ |
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出版タイプ |
AM |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
23H02174 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K26867/ |
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研究課題名 |
酵母小胞体膜上でのmRNA共局在のメカニズム解明と有用蛋白質産生への応用 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
22K19135 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19135/ |
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研究課題名 |
酵母小胞体の形状およびサイズ変化による分泌蛋白質生産能の向上 |
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