| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-12-08 |
| タイトル |
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タイトル |
Potential of a constitutive-UPR and histone deacetylase A-deficient Saccharomyces cerevisiae strain for biomolecule production |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
unfolded protein response |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
protein secretion |
| キーワード |
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主題Scheme |
Other |
|
主題 |
endoplasmic reticulum |
| キーワード |
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主題Scheme |
Other |
|
主題 |
yeast |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
木俣, 有紀
Nguyen, Phuong Thi Mai
Sugimoto, Maya
木俣, 行雄
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The endoplasmic reticulum (ER) is an organelle responsible for the production of secretory proteins and lipid molecules. In many ascomycetous yeasts, including Saccharomyces cerevisiae, HAC1 mRNA undergoes splicing to yield HAC1i mRNA (“i” denotes induced), which is translated into the nuclear transcription factor Hac1 upon ER dysfunction. The extensive transcriptome change triggered by Hac1 is called the unfolded protein response (UPR), which leads to ER enforcement. As an unregulated UPR continuously elevates ER activity and size, S. cerevisiae cells exhibit increased production of secretory proteins and lipid molecules when Hac1 is constitutively and artificially expressed. While this technique holds promise for industrial bioproduction, it has a notable drawback, as cells strongly expressing Hac1 exhibit slow growth, making it difficult to handle and store them. To address this issue, in this study, we searched for fast-growing mutants of HAC1i cells carrying the HAC1i sequence at the HAC1 locus to express Hac1 constitutively. We found that histone deacetylase A (HDA)-deficient mutations, including Δhda3, accelerated the growth of Hac1-expressing cells. Despite their increased growth rate, Δhda3HAC1i cells showed stronger expression of prominent UPR target genes related to ER function than HAC1i cells. Moreover, Δhda3HAC1i cells carried a structurally complex and expanded ER and exhibited high-yield production of triglycerides and of different heterologous model biomolecules, β-carotene and Taka amylase A. In conclusion, we propose that combining artificial Hac1 expression with mutations that affect the chromosomal status holds promise for optimizing yeast-based bioproduction systems. |
| 書誌情報 |
en : Applied and Environmental Microbiology
巻 91,
号 9,
ページ数 18,
発行日 2025-09-17
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| 出版者 |
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出版者 |
American Society for Microbiology |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1098-5336 |
| 出版者版DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1128/aem.00644-25 |
| 出版者版URI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://journals.asm.org/doi/full/10.1128/aem.00644-25 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
Copyright © 2025 Ishiwata-Kimata et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| 著者版フラグ |
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出版タイプ |
VoR |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
23H02174 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K26867/ |
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研究課題名 |
酵母小胞体膜上でのmRNA共局在のメカニズム解明と有用蛋白質産生への応用 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
22K19135 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19135/ |
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研究課題名 |
酵母小胞体の形状およびサイズ変化による分泌蛋白質生産能の向上 |
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