| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-07 |
| タイトル |
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タイトル |
Genomic insights into the carbohydrate-active enzymes of Trichoderma asperellum USM SD4 for potential efficient biomass degradation |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
biomass degradation |
| キーワード |
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主題Scheme |
Other |
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主題 |
carbohydrate-active enzymes |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Trichoderma asperellum USM SD4 |
| キーワード |
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主題Scheme |
Other |
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主題 |
whole-genome sequencing |
| キーワード |
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主題Scheme |
Other |
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主題 |
xylanase and cellulase |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Lee, Chee Keong
Chek, Min Fey
Chong, Jia Fong
Tan, Zhen Wei
吉田, 昭介
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Aims: This study aimed to analyze the Trichoderma asperellum USM SD4 genome to identify carbohydrate-active enzymes (CAZymes) involved in lignocellulosic biomass degradation. These enzymes, particularly glycoside hydrolases (GHs), are crucial for converting biomass into simple sugars. Methodology and results: The genomic DNA of T. asperellum USM SD4 was successfully sequenced, resulting in an assembled genome size of 35.83 Mb containing 10,208 protein-coding sequences. The CAZyme analysis revealed a total of 482 annotated polypeptides, with the highest 54% classified as GHs. The 180 proteins were lignocellulosic CAZymes, with 44% targeting hemicellulose hydrolysis, 42% for cellulose degradation, and 14% for lignin degradation. The present study identifies 14 xylanases and 23 cellulases protein-coding genes in the T. asperellum USM SD4 genome. The secretome analysis revealed 770 proteins, with 182 proteins associated with CAZymes. Among these, one putative xylanase and one putative cellulase were selected for the in vitro characterization. Conclusion, significance and impact of study: This study highlighted the potential of T. asperellum USM SD4 for industrial applications that require efficient biomass degradation. |
| 書誌情報 |
en : Malaysian Journal of Microbiology
巻 21,
号 2,
p. 190-204,
ページ数 15,
発行日 2025-04-01
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| 出版者 |
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出版者 |
Malaysian Society for Microbiology |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2231-7538 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.21161/mjm.240827 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://mjm.usm.my/uploads/issues/2053/EV%20Formatted%20-MJM2-24-0827-Colour%20Ready.pdf |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc/4.0/ |
|
権利情報 |
Malaysian Journal of Microbiology adapts to the Creative Commons, CC BY-NC 4.0 license in publishing open access articles. Under this license, anyone may copy, redistribute and reuse the articles for any non-commercial purposes, as long as the author and the original source of the article are properly cited. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
The Matsumae International Foundation (MIF) |
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研究課題番号 |
23G11 |
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研究課題名 |
2023 MIF Fellowship |
