| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-07 |
| タイトル |
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|
タイトル |
Mapping Morphine's Antinociceptive Impact on the Ventral Tegmental Area During Nociceptive Stimulation: A Novel Microimaging Approach in a Neuropathic Pain Model |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
chronic pain |
| キーワード |
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主題Scheme |
Other |
|
主題 |
neuropathic pain |
| キーワード |
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主題Scheme |
Other |
|
主題 |
partial nerve ligation model |
| キーワード |
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主題Scheme |
Other |
|
主題 |
morphine |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
dopamine |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
VTA |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
GABA |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
CMOS imaging |
| キーワード |
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主題Scheme |
Other |
|
主題 |
GCaMP6 |
| キーワード |
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主題Scheme |
Other |
|
主題 |
fluorescence imaging |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Ganaway, Austin
Kamata, Airi
Yao, Dunyan
Sakoori, Kazuto
岡田, 竜馬
Chen, Ting
Ohta, Yasumi
太田, 淳
Ohsawa, Masahiro
Akay, Metin
Akay, Yasemin M.
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The neurobiology of chronic pain is complex and multifaceted, intertwining with the mesocorticolimbic system to regulate the behavioral and perceptional response to adverse stimuli. Specifically, the ventral tegmental area (VTA), the dopaminergic hub of the reward pathways located deep within the midbrain, is crucial for regulating the release of dopamine (DA) throughout the central nervous system (CNS). To better understand the nuances among chronic pain, VTA response, and therapeutics, implementing progressive approaches for mapping and visualizing the deep brain in real time during nociceptive stimulation is crucial. In this study, we utilize a fluorescence imaging platform with a genetically encoded calcium indicator (GCaMP6s) to directly visualize activity in the VTA during acute nociceptive stimulation in both healthy adult mice and adult mice with partial nerve ligation (PNL)-induced neuropathic pain. We also investigate the visualization of the analgesic properties of morphine. Deep brain imaging using our self-fabricated µ-complementary metal–oxide–semiconductor (CMOS) imaging device allows the tracking of the VTA’s response to adverse stimuli. Our findings show that nociceptive stimulation is associated with a reduction in VTA fluorescence activity, supporting the potential of this platform for visualizing pain-related responses in the central nervous system. Additionally, treatment with morphine significantly reduces the neuronal response caused by mechanical stimuli and is observable using the CMOS imaging platform, demonstrating a novel way to potentially assess and treat neuropathic pain. |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 26,
号 13,
ページ数 18,
発行日 2025-07-07
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| 出版者 |
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出版者 |
MDPI |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1422-0067 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/ijms26136526 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://www.mdpi.com/1422-0067/26/13/6526 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
University of Houston |
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研究課題番号 |
G-53191 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
23H05450 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H05450/ |
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研究課題名 |
早期診断と治療を一元化する埋植型光電子デバイス ～光電気薬学創成に向けて～ |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
24K02786 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K02786/ |
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研究課題名 |
周期的脳活動に着目した痛みの慢性化機構の解明と慢性疼痛緩和療法の開発への応用 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
22KK0180 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KK0180/ |
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研究課題名 |
疼痛の慢性化を予防・改善する脳活動ネットワークの同定と治療への応用 |
