Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics

Chek, Min Fey; Kim, Sun-Yong; Mori, Tomoyuki; Matsumoto, Keiji; Sato, Shunsuke; 箱嶋, 敏雄; ハコシマ, トシオ; Hakoshima, Toshio

doi:https://doi.org/10.1002/anie.202504626

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インデックスツリー

アイテム

Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics

http://hdl.handle.net/10061/0002001254

アイテムタイプ

学術雑誌論文 / Journal Article(1)

公開日

2025-10-30

タイトル

Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics

言語

eng

キーワード

主題Scheme

Other

主題

Biodegradable plastic

キーワード

主題Scheme

Other

主題

Egress tunnel

キーワード

主題Scheme

Other

主題

Enzyme mechanisms

キーワード

主題Scheme

Other

主題

Full-length structure

キーワード

主題Scheme

Other

主題

PHA synthase

資源タイプ

journal article

アクセス権

open access

著者

Chek, Min Fey
Kim, Sun-Yong
Mori, Tomoyuki
Matsumoto, Keiji
Sato, Shunsuke
箱嶋, 敏雄

ja	箱嶋, 敏雄
ja-Kana	ハコシマ, トシオ
en	Hakoshima, Toshio

Search repository

抄録

内容記述タイプ

Abstract

内容記述

Polyhydroxyalkanoate (PHA) is a biodegradable polyester that can serve as a promising alternative to petrochemical plastics, which present a serious source of pollution. PHA synthase (PhaC) is a key enzyme responsible for producing a wide variety of PHAs in microorganisms. Here, we present crystal structures of full-length PhaC from Aeromonas caviae, a high-performance PhaC employed for industrial use. The structure reveals an N-terminal helical domain that mediates head-to-head dimerization and stabilizes the C-terminal α/β catalytic domain to form a tunnel that connects the catalytic center embedded inside the protein to the protein surface. We showed that this tunnel is a putative egress tunnel for the product PHA chain. Our results establish a fundamental understanding of the PhaC machinery that should lead to improvement of this enzyme in industrial applications.

書誌情報

en : Angewandte Chemie International Edition

巻 64, 号 26, ページ数 15, 発行日 2025-06-24

出版者

John Wiley & Sons, Ltd

ISSN

収録物識別子タイプ

EISSN

収録物識別子

1521-3773

出版者版DOI

権利

権利情報Resource

https://creativecommons.org/licenses/by-nc-nd/4.0/

権利情報

© 2025 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. This is an open access article underthe terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in anymedium, provided the original work is properly cited, the use isnon-commercial and no modiﬁcations or adaptations are made.

著者版フラグ

出版タイプ

助成情報

助成機関名

Japan Science and Technology Agency (JST)

研究課題番号

29A1027

研究課題名

A-STEP

助成情報

助成機関名

New Energy and IndustrialTechnology Development Organization (NEDO)

研究課題番号

P20005

助成情報

助成機関名

Takeda Science Foundation

助成情報

助成機関名

Japan Society for the Promotion of Science (JSPS)

研究課題番号

21K15028

研究課題番号URI

https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21K15028/

研究課題名

Structure-guided engineering of a biodegradable plastic synthase for tailor-made polymer production

助成情報

助成機関名