| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-15 |
| タイトル |
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タイトル |
Wnt-dependent mechanism of the apical constriction of roof plate cells in developing mouse spinal cord |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Wnt |
| キーワード |
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主題Scheme |
Other |
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主題 |
roof plate |
| キーワード |
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主題Scheme |
Other |
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主題 |
neural tube |
| キーワード |
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主題Scheme |
Other |
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主題 |
apical constriction |
| キーワード |
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主題Scheme |
Other |
|
主題 |
morphogenesis |
| キーワード |
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主題Scheme |
Other |
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主題 |
cytoskeleton |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Shinozuka, Takuma
Okubo, Tadashi
笹井, 紀明
Takada, Shinji
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Apical constriction of epithelial cells usually occurs in a local portion of epithelial sheet, which results in bending of epithelial tissues. However, it is uncertain whether diffusible signal molecules, like Wnt, regulate such locally restricted events. Here, we show that Wnt ligands are required for apical constriction of Wnt1-expressing roof plate (RP) cells during development of the neural tube. Analysis of Wntless conditional knock-out (cKO) embryos, in which Wnt secretion from Wnt1-expressing roof plate cells is impaired, revealed that RP-derived Wnt ligands are required for phosphorylation of myosin light chain (MLC) and apical constriction of RP cells. Loss- or gain-of-function analysis of β-catenin reveals that this apical constriction is regulated in a β-catenin-dependent manner. Consistent with the timing of apical constriction, Wnt ligands accumulate on the apical side of RP cells. In embryos with Wnt1-expressing RP-specific defects in synthesis of heparan sulfate proteoglycan, apical accumulation of Wnt ligands and apical constriction are impaired. Therefore, we propose that specific accumulation of Wnt ligands on RP cells drives apical constriction of these cells. |
| 書誌情報 |
en : Frontiers in Cell and Developmental Biology
巻 13,
ページ数 9,
発行日 2025-07-07
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| 出版者 |
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出版者 |
Frontiers Media |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2296-634X |
| 出版者版DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3389/fcell.2025.1571770 |
| 出版者版URI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
URI |
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関連識別子 |
https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1571770/full |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
© 2025 Shinozuka, Okubo, Sasai and Takada. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| 著者版フラグ |
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出版タイプ |
VoR |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
18H02454 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02454/ |
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研究課題名 |
脊髄神経管発生をモデルにしたWntの空間分布制御機構の研究 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
21H02498 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02498/ |
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研究課題名 |
Wntリガンドの動態計測に基づく組織発生機構の解析 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
24111002 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-24111002/ |
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研究課題名 |
リンパ器官形成の分子機構と制御 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
17H05782 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-17H05782/ |
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研究課題名 |
シグナルによる発生時計の制御のしくみとその意義 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
19H04797 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-19H04797/ |
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研究課題名 |
体節形成における分節時計と空間情報の相互作用の解析 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
20K15809 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K15809/ |
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研究課題名 |
細胞の形態変化におけるWntタンパク質の役割 |
| 助成情報 |
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助成機関名 |
National Institutes of Natural Sciences |
fulltext (9.3 MB)
