| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-29 |
| タイトル |
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タイトル |
Comparison of the Metabolite Profiles of HT-29 Colorectal Cancer Cells Treated with Curcumin, Cisplatin, 5-Fluorouracil and Doxorubicin in a Metabolomic Approach |
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Metabolomics |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Colorectal Cancer |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Curcumin |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Arsianti, Ade
Widiasti, Innas
Tedjo, Aryo
廣田, 俊
Tanimoto, Hiroki
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Chemotherapy for colorectal cancer often leads to significant adverse effects on patients, underscoring the need for alternative treatments. Herbal medicines like curcumin are considered a valuable complementary therapy due to their low toxicity profile and potential to mitigate the side effects of chemotherapy. Curcumin's mechanism of action targets multiple pathways, with untargeted metabolomic analysis helping to understand its exact mechanisms and subsequent treatment response. The aim of this study was to compare HT-29 cancer cell metabolites after curcumin and chemotherapy drug interventions to identify metabolites that can predict similar mechanisms of action between these treatments. Principal Component Analysis (PCA) of Fourier transform infrared spectroscopy (FTIR) absorption spectrum showed similar metabolite profiles in HT-29 cell culture media treated with curcumin and the chemotherapeutic cisplatin. Five cell metabolomes emerged after additional gas chromatography mass spectrometry/mass spectrometry (GC-MS/MS) and MS-DIAL data annotation: 1-bromo-2-chloroethane, 2-cyanoacetamide, dimethylamine (DMA), 2-nitrobenzo acid, and butane. The confusion matrix of these five annotated metabolites could be distinguished in HT-29 cell cultures treated with curcumin, but not in control cell cultures or those treated with the drugs cisplatin, doxorubicin, or 5-fluorouracil (5-FU). 2-cyanoacetamide in particular can be used as a marker of HT-29 cells' response to treatment with curcumin based on a p-value of < 0.05. According to these findings, no metabolite can predict the resemblance of curcumin's mechanism of action to chemotherapeutic medicines. Further study should therefore focus on in vivo experimental validation and upgrading metabolomic analysis technologies to further establish the similarities in the metabolite profiles of curcumin and cisplatin treatments. |
| 書誌情報 |
en : Tropical Journal of Natural Product Research
巻 9,
号 3,
p. 1179-1186,
ページ数 8,
発行日 2025-03-28
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| 出版者 |
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出版者 |
Natural Product Research group, University of Benin |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2616-0692 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.26538/tjnpr/v9i3.38 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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|
関連識別子 |
https://www.tjnpr.org/index.php/home/article/view/6053 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2025 Arsianti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Universitas Indonesia |
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|
研究課題番号 |
NKB-126/UN2.RST/HKP.05.00/2022 |
