| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-25 |
| タイトル |
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タイトル |
Exportin-5 binding precedes 5′- and 3′-end processing of tRNA precursors in Drosophila |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
RNA export |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Drosophila |
| キーワード |
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主題Scheme |
Other |
|
主題 |
exportin-5 |
| キーワード |
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主題Scheme |
Other |
|
主題 |
tRNA processing |
| キーワード |
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主題Scheme |
Other |
|
主題 |
CLIP |
| キーワード |
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主題Scheme |
Other |
|
主題 |
intronless gene |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Li, Ze
Iida, Junko
椎森, 仁美
岡村, 勝友
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Exportin5 (Exp5) is the major miRNA nuclear export factor and recognizes structural features of pre-miRNA hairpins, while it also exports other minihelix-containing RNAs. In Drosophila, Exp5 is suggested to play a major role in tRNA export because the gene encoding the canonical tRNA export factor Exportin-t is missing in its genome. To understand molecular functions of fly Exp5, we studied the Exp5/RNA interactome in the cell line S2R + using the crosslinking and immunoprecipitation (CLIP) technology. The CLIP experiment captured known substrates such as tRNAs and miRNAs and detected candidates of novel Exp5 substrates including various mRNAs and long non-coding RNAs (lncRNAs). Some mRNAs and lncRNAs enriched PAR-CLIP tags compared to their expression levels, suggesting selective binding of Exp5 to them. Intronless mRNAs tended to enrich PAR-CLIP tags; therefore, we proposed that Exp5 might play a role in the export of specific classes of mRNAs/lncRNAs. This result suggested that Drosophila Exp5 might have a wider variety of substrates than initially thought. Surprisingly, Exp5 CLIP reads often contained sequences corresponding to the flanking 5′-leaders and 3′-trailers of tRNAs, which were thought to be removed prior to nuclear export. In fact, we found pre-tRNAs before end-processing were present in the cytoplasm, supporting the idea that tRNA end-processing is a cytoplasmic event. In summary, our results provide a genome-wide list of Exp5 substrate candidates and suggest that flies may lack a mechanism to distinguish pre-tRNAs with or without the flanking sequences. |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 300,
号 9,
ページ数 11,
発行日 2024-09-01
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1083-351X |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jbc.2024.107632 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.jbc.org/article/S0021-9258(24)02133-1/fulltext |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
© 2024 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
National Institutes of Health (NIH) |
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研究課題番号 |
2P40OD010949 |
| 助成情報 |
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助成機関名 |
National Research Foundation, Prime Minister’s Office, Singapore (NRF) |
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研究課題番号 |
NRF2011NRF-NRFF001-042 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
17K20145 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K20145/ |
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研究課題名 |
ｍｉＲＮＡ生合成の調節機構とその影響のゲノムワイド解析 |
| 助成情報 |
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助成機関名 |
Takeda Science Foundation |
| 助成情報 |
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助成機関名 |
Ohsumi Frontier Science Foundation |
