| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-24 |
| タイトル |
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タイトル |
Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
BAM complex |
| キーワード |
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主題Scheme |
Other |
|
主題 |
BepA |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
disulfide isomerization |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
β-barrel membrane protein |
| キーワード |
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主題Scheme |
Other |
|
主題 |
protein folding |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
宮﨑, 亮次
Kimoto, Mai
甲賀, 栄貴
塚崎, 智也
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Gram-negative bacteria possess an outer membrane (OM) that acts as a barrier against toxic compounds. Lipopolysaccharide (LPS) in the outer leaflet of the OM is crucial for barrier function. After its synthesis in the cytoplasm and translocation to the periplasm, LPS is transported to the OM by the LPS transport system. The LPS translocon, composed of an OM protein LptD and a lipoprotein LptE, mediates LPS assembly into the OM. Recently, the small lipoprotein LptM (YifL) was identified as a novel LptD/E-interactor that facilitates LptD maturation. However, its mechanism remains unclear. Here, we investigated the detailed interaction between LptM and LptD. We found that LptM interacts with the folded LptD intermediate at the late stage of its maturation. Mutational analyses demonstrated that the N-terminal conserved region (C20GLKGPLYF28) of LptM is essential for its function. Cryo-EM structural analysis of the E. coli LptD/E/M complex, combined with biochemical analyses, revealed the molecular basis of the LptM–LptD interaction and its functional importance. Thus, we propose that LptM functions as a “barrel-rivet,” stabilizing LptD for its proper assembly. |
| 書誌情報 |
en : Cell Reports
巻 44,
号 8,
ページ数 17,
発行日 2025-07-16
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| 出版者 |
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出版者 |
Cell Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2211-1247 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.celrep.2025.116013 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.sciencedirect.com/science/article/pii/S2211124725007843?via%3Dihub |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
