| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-24 |
| タイトル |
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タイトル |
Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
iron transport |
| キーワード |
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主題Scheme |
Other |
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主題 |
Fec system |
| キーワード |
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主題Scheme |
Other |
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主題 |
Ton system |
| キーワード |
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主題Scheme |
Other |
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主題 |
twin-arginine translocation system |
| キーワード |
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主題Scheme |
Other |
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主題 |
signal transduction |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Yokoyama, Tatsuhiko
宮﨑, 亮次
Suzuki, Takehiro
Dohmae, Naoshi
Nagai, Hiroki
塚崎, 智也
Kubori, Tomoko
Akiyama, Yoshinori
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
TonB-dependent signal transduction is a versatile mechanism observed in gram-negative bacteria, integrating energy-dependent substrate transport with signal relay. In Escherichia coli, the TonB-ExbBD motor complex energizes the TonB-dependent transporter FecA, facilitating ferric citrate import. FecA also functions as a sensor, transmitting signals to the cytoplasmic membrane protein FecR. We previously demonstrated that FecR undergoes a three-step cleavage process, culminating in the activation of the cytoplasmic sigma factor FecI, which drives fec gene transcription. Here, we describe the complete mechanism of FecR cleavage-mediated ferric citrate signaling involving FecA and TonB. The cleavage cascade begins with FecR autoproteolysis prior to membrane integration. The soluble C-terminal domain (CTD) fragment of FecR is co-translocated with the N-terminal domain (NTD) fragment through a Tat system-mediated process. In the periplasm, the interaction between the CTD and NTD fragments prevents further cleavage. This inhibition is lifted by TonB-mediated motor function, which releases the CTD, allowing the cleavage cascade to proceed. This process is essential for ferric citrate signal-induced activation of fec gene expression. Our findings reveal that the regulation of FecR cleavage, relying on the TonB-FecA axis, plays a central role in bacterial response to ferric citrate signals. |
| 書誌情報 |
en : Proceedings of the National Academy of Sciences
巻 122,
号 16,
ページ数 10,
発行日 2025-04-22
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| 出版者 |
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出版者 |
National Academy of Sciences |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1073/pnas.2500366122 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.pnas.org/doi/10.1073/pnas.2500366122 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
© 2025 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial- NoDerivatives License 4.0 (CC BY-NC- ND). |
| 著者版フラグ |
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出版タイプ |
NA |
