| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-16 |
| タイトル |
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タイトル |
Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can induce chromosome elimination in the fission yeast Schizosaccharomyces pombe |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Cdc48 |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Chromosome |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Cnp1 |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Ufd1 |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Centromere |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Pombe |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
中瀬, 由起子
Murakami, Hiroaki
Suma, Michiko
Nagano, Kaho
Wakuda, Airi
Kitagawa, Teppei
Matsumoto, Tomohiro
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
CENP-A determines the identity of the centromere. Because the position and size of the centromere and its number per chromosome must be maintained, the distribution of CENP-A is strictly regulated. In this study, we have aimed to understand mechanisms to regulate the distribution of CENP-A (Cnp1SP) in fission yeast. A mutant of the ufd1+ gene (ufd1-73) encoding a cofactor of Cdc48 ATPase is sensitive to Cnp1 expressed at a high level and allows mislocalization of Cnp1. The level of Cnp1 in centromeric chromatin is increased in the ufd1-73 mutant even when Cnp1 is expressed at a normal level. A preexisting mutant of the cdc48+ gene (cdc48-353) phenocopies the ufd1-73 mutant. We have also shown that Cdc48 and Ufd1 proteins interact physically with centromeric chromatin. Finally, Cdc48 ATPase with Ufd1 artificially recruited to the centromere of a mini-chromosome (Ch16) induce a loss of Cnp1 from Ch16, leading to an increased rate of chromosome loss. It appears that Cdc48 ATPase, together with its cofactor Ufd1 remove excess Cnp1 from chromatin, likely in a direct manner. This mechanism may play a role in centromere disassembly, a process to eliminate Cnp1 to inactivate the kinetochore function during development, differentiation, and stress response. |
| 書誌情報 |
en : Biology Open
巻 13,
号 4,
ページ数 9,
発行日 2024-04-08
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| 出版者 |
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出版者 |
The Company of Biologists |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2046-6390 |
| 出版者版DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1242/bio.060287 |
| 出版者版URI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
URI |
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関連識別子 |
https://journals.biologists.com/bio/article/13/4/bio060287/346490 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
© 2024. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| 著者版フラグ |
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出版タイプ |
VoR |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
20K06485 |
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研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K06485/ |
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研究課題名 |
セントロメア崩壊：その機序の解明 |
| 助成情報 |
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助成機関名 |
Kyoto University |
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