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N-terminal truncation of PhaCBP-M-CPF4 and its effect on PHA production
http://hdl.handle.net/10061/0002001112
http://hdl.handle.net/10061/00020011120cf2a589-8262-4459-a6d9-84142ec47ccc
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||
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| 公開日 | 2025-08-08 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | N-terminal truncation of PhaCBP-M-CPF4 and its effect on PHA production | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Polyhydroxyalkanoate synthase | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | N-terminal truncation | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Molecular weights | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | phaC gene expression | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | PHA granule morphology | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 著者 |
Neoh, Soon Zher
× Neoh, Soon Zher
× Tan, Hua Tiang
× Trakunjae, Chanaporn
× Chek, Min Fey
× Vaithanomsat, Pilanee
× 箱嶋, 敏雄
× Sudesh, Kumar
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| 抄録 | ||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||
| 内容記述 | Background Among the polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] is reported to closely resemble polypropylene and low-density polyethylene. Studies have shown that PHA synthase (PhaC) from mangrove soil (PhaCBP-M-CPF4) is an efficient PhaC for P(3HB-co-3HHx) production and N-termini of PhaCs influence its substrate specificity, dimerization, granule morphology, and molecular weights of PHA produced. This study aims to further improve PhaCBP-M-CPF4 through N-terminal truncation. Results The N-terminal truncated mutants of PhaCBP-M-CPF4 were constructed based on the information of the predicted secondary and tertiary structures using PSIPRED server and AlphaFold2 program, respectively. The N-terminal truncated PhaCBP-M-CPF4 mutants were evaluated in C. necator mutant PHB$22124 based on the cell dry weight, PHA content, 3HHx molar composition, molecular weights, and granule morphology of the PHA granules. The results showed that most transformants harbouring the N-terminal truncated PhaCBP-M-CPF4 showed a reduction in PHA content and cell dry weight except for PhaCBP-M-CPF4 G8. PhaCBP-M-CPF4 G8 and A27 showed an improved weight-average molecular weight (Mw) of PHA produced due to lower expression of the truncated PhaCBP-M-CPF4. Transformants harbouring PhaCBP-M-CPF4 G8, A27, and T74 showed a reduction in the number of granules. PhaCBP-M-CPF4 G8 produced higher Mw PHA in mostly single larger PHA granules with comparable production as the full-length PhaCBP-M-CPF4. Conclusion This research showed that N-terminal truncation had effects on PHA accumulation, substrate specificity, Mw, and granule morphology. This study also showed that N-terminal truncation of the amino acids that did not adopt any secondary structure can be an alternative to improve PhaCs for the production of PHA with higher Mw in mostly single larger granules. |
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| 書誌情報 |
en : Microbial Cell Factories 巻 23, ページ数 16, 発行日 2024-02-15 |
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| 出版者 | ||||||||||||||||||||||||
| 出版者 | BioMed Central | |||||||||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||
| 収録物識別子 | 1475-2859 | |||||||||||||||||||||||
| 出版者版DOI | ||||||||||||||||||||||||
| 関連タイプ | isReplacedBy | |||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1186/s12934-024-02329-w | |||||||||||||||||||||||
| 出版者版URI | ||||||||||||||||||||||||
| 関連タイプ | isReplacedBy | |||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||
| 関連識別子 | https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-024-02329-w | |||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||||||||
| 権利情報 | $00A9 The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | |||||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||||
| 出版タイプ | NA | |||||||||||||||||||||||
| 助成情報 | ||||||||||||||||||||||||
| 助成機関名 | Universiti Sains Malaysia (USM) | |||||||||||||||||||||||
| 助成情報 | ||||||||||||||||||||||||
| 助成機関名 | Ministry of Higher Education | |||||||||||||||||||||||
| 研究課題名 | Soil Analysis and Value-Addition to Oil Palm Trunk (OPT) and sap through Biotechnology | |||||||||||||||||||||||
| 助成情報 | ||||||||||||||||||||||||
| 助成機関名 | Japan Science and Technology Agency (JST) | |||||||||||||||||||||||
| 研究課題番号 | 203/PBIOLOGI/67811001 | |||||||||||||||||||||||
| 研究課題名 | Science and Technology Research Partnership for Sustainable Development (SATREPS) | |||||||||||||||||||||||
| 助成情報 | ||||||||||||||||||||||||
| 助成機関名 | Zacros (Fujimori Kogyo Co. Ltd.) | |||||||||||||||||||||||
| 研究課題番号 | 304/PBIOLOGI/6501281/F117 | |||||||||||||||||||||||
