| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-07-03 |
| タイトル |
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タイトル |
Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Lim, Jonathan Jun-Yong
Murata, Yamato
由利, 俊祐
Kitamuro, Kohei
河合, 太郎
磯谷, 綾子
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Gene-knockout animal models with organ-deficient phenotypes used for blastocyst complementation are generally not viable. Animals need to be maintained as heterozygous mutants, and homozygous mutant embryos yield only one-fourth of all embryos. In this study, we generated organ-deficient embryos using the CRISPR-Cas9-sgRNAms system that induces cell death with a single-guide RNA (sgRNAms) targeting multiple sites in the genome. The Cas9-sgRNAms system interrupted cell proliferation and induced cell ablation in vitro. The mouse model had Cas9 driven by the Foxn1 promoter with a ubiquitous expression cassette of sgRNAms at the Rosa26 locus (Foxn1Cas9; Rosa26_ms). It showed an athymic phenotype similar to that of nude mice but was not hairless. Eventually, a rat cell-derived thymus in an interspecies chimera was generated by blastocyst complementation of Foxn1Cas9; Rosa26_ms mouse embryos with rat embryonic stem cells. Theoretically, a half of the total embryos has the Cas9-sgRNAms system because Rosa26_ms could be maintained as homozygous. |
| 書誌情報 |
en : Scientific Reports
巻 14,
号 1,
ページ数 12,
発行日 2024-05-09
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| 出版者 |
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出版者 |
Nature Research |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2045-2322 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-024-61167-3 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41598-024-61167-3 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
$00A9 The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creativecommons.org/licenses/by/4.0/. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
21K19267 |
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研究課題名 |
異種ハイブリッド胎盤を用いた母子間認識の研究 |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
23K18577 |
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研究課題名 |
胎児の臓器を立体構造を保持したまま成体内で育てる研究 |
| 助成情報 |
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助成機関名 |
KAC 40th Anniversary Research Grant |
| 助成情報 |
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助成機関名 |
NOVARTIS Foundation (Japan) for the Promotion of Science Grant |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
22K06067 |
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研究課題名 |
組織特異的な細胞競合力の調節による異種臓器作製への試み |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
18K06031 |
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研究課題名 |
新規臓器欠損法による異種キメラ動物を利用した様々な臓器の作成 |
