| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-26 |
| タイトル |
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タイトル |
An improved method for the highly specific detection of transcription start sites |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
Transcriptome Mapping - Monitoring Gene Expression |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Seki, Masahide
Kuze, Yuta
Zhang, Xiang
Kurotani, Ken-ichi
Notaguchi, Michitaka
Nishio, Haruki
Kudoh, Hiroshi
Suzaki, Takuya
吉田, 聡子
Sugano, Sumio
Matsushita, Tomonao
Suzuki, Yutaka
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Precise detection of the transcriptional start site (TSS) is a key for characterizing transcriptional regulation of genes and for annotation of newly sequenced genomes. Here, we describe the development of an improved method, designated ‘TSS-seq2.’ This method is an iterative improvement of TSS-seq, a previously published enzymatic cap-structure conversion method to detect TSSs in base sequences. By modifying the original procedure, including by introducing split ligation at the key cap-selection step, the yield and the accuracy of the reaction has been substantially improved. For example, TSS-seq2 can be conducted using as little as 5 ng of total RNA with an overall accuracy of 96%; this yield a less-biased and more precise detection of TSS. We then applied TSS-seq2 for TSS analysis of four plant species that had not yet been analyzed by any previous TSS method. |
| 書誌情報 |
en : Nucleic Acids Research
巻 52,
号 2,
発行日 2024-01-25
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
0305-1048 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/nar/gkad1116 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://academic.oup.com/nar/article/52/2/e7/7442529 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
$00A9 The Author(s) 2023. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP20H05905 |
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研究課題名 |
不均一環境変動に対する植物のレジリエンスを支える多層的情報統御の分子機構 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP20H05906 |
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研究課題名 |
植物の不均一環境変動へのレジリエンスを支える転写開始点制御機構 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP20H05909 |
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研究課題名 |
不均一土壌環境に応答した寄生植物の感染統御機構 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP20H05908 |
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研究課題名 |
複合的な不均一環境における根粒共生を介した窒素栄養獲得の統御機構 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP21H04977 |
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研究課題名 |
変動環境下での頑健な応答を支える長期クロマチン記憶 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP21H05657 |
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研究課題名 |
不均一な環境下で全身移行するmRNAに関する研究 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP21H05659 |
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研究課題名 |
不規則な環境変動下における花成抑制遺伝子FLCの頑健な制御 |
| 助成情報 |
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology(MEXT) |
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研究課題番号 |
JP22H04925 |
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研究課題名 |
先進ゲノム解析研究推進プラットフォーム |
