| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-23 |
| 日付 |
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日付 |
2026-05-14 |
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日付タイプ |
Available |
| タイトル |
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タイトル |
Construction of a Cyclic Regular-Triangle Trimer of Cytochrome c555 with a Central Hole Using Sortase A |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
cyclic protein |
| キーワード |
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主題Scheme |
Other |
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主題 |
cytochrome c555 |
| キーワード |
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主題Scheme |
Other |
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主題 |
nanoporous structure |
| キーワード |
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主題Scheme |
Other |
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主題 |
sortase A |
| キーワード |
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主題Scheme |
Other |
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主題 |
supramolecular protein |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
embargoed access |
| 著者 |
Novientri, Gissi
Fujiwara, Kodai
真島, 剛史
Matsuura, Hiroaki
Ogata, Hideaki
Uchihashi, Takayuki
Fujii, Sotaro
Sambongi, Yoshihiro
廣田, 俊
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Protein-based supramolecules require precise arrangement of building blocks. A regular-triangle trimer (cp-c555)3 has been constructed using an α-helix-inserted-circular permutant (cp-c555) of Aquifex aeolicus cytochrome (cyt) c555, where the trimers may dissociate to monomers. In this study, we stabilized the regular-triangle structure by constructing a cyclic regular-triangle of three α-helix-linked cyt c555 molecules using sortase-mediated ligation (SML). Comparing SML using sortase A for six cp-c555 variant trimers, the variant with GGG at the N-terminus and LPETG at the C-terminus reacted most efficiently. OP-(c555)3 was designed, in which two cyt c555 molecules were fused using an α-helix, generating a dimer. The cyt c555 C-terminal region was attached to the N-terminus of the dimer, and the cyt c555 N-terminal region was attached to the C-terminus of the dimer using the same α-helix. OP-(c555)3 was expressed in Escherichia coli cells, and the termini were connected by SML, forming a cyclic regular-triangle, CL-(c555)3. CL-(c555)3 showed higher thermostability than (cp-c555)3 and OP-(c555)3. CL-(c555)3 structural stability was confirmed using high-speed atomic force microscopy. The X-ray crystal structure of CL-(c555)3 showed a cyclic structure and a nanoporous supramolecular assembly. These results demonstrate that a nanoporous supramolecular assembly can be constructed by designing a cyclic molecule with a central hole using SML. |
| 書誌情報 |
en : Chemistry - A European Journal
巻 31,
号 27,
発行日 2025-05-14
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1521-3765 |
| 出版者版DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/chem.202404736 |
| 出版者版URI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
URI |
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関連識別子 |
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202404736 |
| 権利 |
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権利情報 |
$00A9 2025 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Gissi Novientri, Kodai Fujiwara, Tsuyoshi Mashima, Hiroaki Matsuura, Hideaki Ogata, Takayuki Uchihashi, Sotaro Fujii, Yoshihiro Sambongi, Shun Hirota, Construction of a Cyclic Regular-Triangle Trimer of Cytochrome c555 with a Central Hole Using Sortase A. Chemistry - A European Journal 31, 27, which has been published in final form at https://doi.org/10.1002/chem.202404736. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. 出版社許諾条件により、本文は2026年5月14日以降に公開 |
| 著者版フラグ |
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出版タイプ |
AM |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science (JSPS) |
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研究課題番号 |
JP23K21152 |
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研究課題名 |
タンパク質3Dドメインスワッピングの包括的理解と応用 |
| 助成情報 |
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助成機関名 |
Japan Science and Technology Agency(JST), CREST |
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研究課題番号 |
JP20338388 |
fulltext (5.2 MB)
