| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-19 |
| タイトル |
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タイトル |
Region of interest determination algorithm of lensless calcium imaging datasets |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Castillo, Virgil Christian Garcia
Akbar, Latiful
Siwadamrongpong, Ronnakorn
Ohta, Yasumi
Kawahara, Mamiko
Sunaga, Yoshinori
Takehara, Hironari
Tashiro, Hiroyuki
笹川, 清隆
太田, 淳
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Advances in fluorescence imaging technology have been crucial to the progress of neuroscience. Whether it was specific expression of indicator proteins, detection of neurotransmitters, or miniaturization of fluorescence microscopes, fluorescence imaging has improved upon electrophysiology, the gold standard for monitoring brain activity, and enabled novel methods to sense activity in the brain. Hence, we developed a lightweight and compact implantable CMOS-based lensless Ca2+ imaging device for freely moving transgenic G-CaMP mouse experiments. However, without a lens system, determination of regions of interest (ROI) has proven challenging. Localization of fluorescence activity and separation of signal from noise are difficult. In this study, we report an ROI selection method using a series of adaptive binarizations with a gaussian method and morphological image processing. The parameters for each operation such as the kernel size, sigma and footprint size were optimized. We then validated the utility of the algorithm with simulated data and freely moving nociception experiments using the lensless devices. The device was implanted in the dorsal raphe nucleus to observe pain-related brain activity following a formalin test to stimulate pain. We observed significant increases in fluorescence activity after formalin injection compared to the control group when using the ROI determination algorithm. |
| 書誌情報 |
en : PLOS ONE
巻 19,
号 9,
発行日 2024-09-17
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| 出版者 |
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出版者 |
Public Library of Science |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1932-6203 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0308573 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0308573 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
$00A9 2024 Castillo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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助成機関名 |
Japan Society for the Promotion of Science(JSPS) |
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研究課題番号 |
JP18H03780 |
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研究課題名 |
光による生体神経インターフェイスデバイスの研究 |
