| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-20 |
| タイトル |
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タイトル |
Fast-Growing Saccharomyces cerevisiae Cells with a Constitutive Unfolded Protein Response and Their Potential for Lipidic Molecule Production |
| 言語 |
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言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Saccharomyces cerevisiae |
| キーワード |
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主題Scheme |
Other |
|
主題 |
endoplasmic reticulum |
| キーワード |
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主題Scheme |
Other |
|
主題 |
endoplasmic reticulum stress |
| キーワード |
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主題Scheme |
Other |
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主題 |
lipid synthesis |
| キーワード |
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主題Scheme |
Other |
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主題 |
stress response |
| キーワード |
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主題Scheme |
Other |
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主題 |
unfolded protein response |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Nguyen, Phuong Thi Mai
木俣, 有紀
木俣, 行雄
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In Saccharomyces cerevisiae cells, dysfunction of the endoplasmic reticulum (ER), so-called ER stress, leads to conversion of HAC1 mRNA to the spliced form (HAC1i), which is translated into a transcription factor that drastically changes the gene expression profile. This cellular response ultimately enhances ER functions and is named the unfolded protein response (UPR). Artificial evocation of the UPR is therefore anticipated to increase productivity of beneficial materials on and in the ER. However, as demonstrated here, cells constitutively expressing HAC1i mRNA (HAC1i cells), which exhibited a strong UPR even under nonstress conditions, grew considerably slowly and frequently yielded fast-growing and low-UPR progeny. Intriguingly, growth of HAC1i cells was faster in the presence of weak ER stress that was induced by low concentrations of the ER stressor tunicamycin or by cellular expression of the ER-located version of green fluorescent protein (GFP). HAC1i cells producing ER-localized GFP stably exhibited a strong UPR activity, carried a highly expanded ER, and abundantly produced triglycerides and heterogenous carotenoids. We therefore propose that our findings provide a basis for metabolic engineering to generate cells producing valuable lipidic molecules. |
| 書誌情報 |
en : Applied and Environmental Microbiology
巻 88,
号 21,
発行日 2022-10-18
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| 出版者 |
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出版者 |
American Society for Microbiology |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1098-5336 |
| 出版者版DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1128/aem.01083-22 |
| 出版者版URI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
URI |
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関連識別子 |
https://journals.asm.org/doi/10.1128/aem.01083-22 |
| 権利 |
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権利情報 |
$00A9 2022 American Society for Microbiology. |
| 著者版フラグ |
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出版タイプ |
AM |
| 助成情報 |
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助成機関名 |
Noda Institute for Scientific Research |
fulltext (574.6 KB)
