| アイテムタイプ |
会議発表論文 / Conference Paper(1) |
| 公開日 |
2025-05-14 |
| タイトル |
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|
タイトル |
Detection of multiple fluorescent dyes using liquid crystal variable retarder and sparse modeling |
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Fluorescence quantification |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Liquid crystal variable retarder |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Sparse modeling |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Spatial transcriptome analysis |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Spectrum modulator |
| 資源タイプ |
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資源タイプ |
conference paper |
| アクセス権 |
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|
アクセス権 |
open access |
| 著者 |
Fujiwara, Kazuma
舩冨, 卓哉
北野, 和哉
藤村, 友貴
向川, 康博
Ochiai, Hiroshi
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
In spatial transcriptomics, which allows the analysis of gene expression while preserving its location in tissues, RNA molecules are hybridized with a fluorescent-labeled DNA probe for detection. In this study, we aim to improve the efficiency of spatial transcriptomics by simultaneously using multiple fluorescent dyes with overlapping spectra. We propose a method to quantify each fluorescent dyes using a liquid crystal variable retarder as a spectral modulator, which can control the spectral transmittance by changing the voltage. The spectrum of light passing through the modulator is integrated by the image sensor and observed as intensity. We quantify the fluorescent dyes at each pixel using intensities of various spectral transmittances as a spectral code and applying sparse modeling using a dictionary created by simulating observations for the fluorescent dyes used in hybridization. We verified the principle of the proposed method and demonstrated its feasibility through simulation experiments. |
| 書誌情報 |
en : Electronic Imaging
巻 36,
号 15,
p. 161-1-161-5,
発行日 2024-01-21
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| 会議情報 |
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会議名 |
Electronic Imaging 2024 |
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開始年 |
2024 |
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開始月 |
01 |
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開始日 |
21 |
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終了年 |
2024 |
|
|
終了月 |
01 |
|
|
終了日 |
25 |
|
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開催地 |
California |
|
開催国 |
USA |
| 出版者 |
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|
出版者 |
Society for Imaging Science and Technology |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2470-1173 |
| 出版者版DOI |
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|
関連タイプ |
isReplacedBy |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.2352/EI.2024.36.15.COIMG-161 |
| 出版者版URI |
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|
関連タイプ |
isReplacedBy |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://library.imaging.org/ei/articles/36/15/COIMG-161 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| 著者版フラグ |
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出版タイプ |
NA |
| 助成情報 |
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|
助成機関名 |
Japan Science and Technology Agency (JST) |
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|
研究課題番号 |
JPMJPR2025 |
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|
研究課題名 |
パースモデリングによる幾何変換場のモデル化 |
| 助成情報 |
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|
助成機関名 |
Japan Science and Technology Agency (JST) |
|
|
研究課題番号 |
JPMJCR23N3 |
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|
研究課題名 |
マルチモーダル時空間統合オミクス解析による哺乳類細胞運命制御基盤の理解 |
