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EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells
http://hdl.handle.net/10061/0002000792
http://hdl.handle.net/10061/0002000792f18b96ac-ddb0-4dcf-b1eb-86a3f88d2c04
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2025-02-28 | |||||||||||
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| タイトル | EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | EPHA2 | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | pluripotent stem cells | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | cell surface marker | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | tumorigenesis | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | stem cell transplantation | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 著者 |
印東, 厚
× 印東, 厚× Watanabe-Susaki, Kanako
× Kato, Taku
× Kiritani, Hibiki
× 栗崎, 晃 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) possess the intrinsic ability to differentiate into diverse cellular lineages, marking them as potent instruments in regenerative medicine. Nonetheless, the proclivity of these stem cells to generate teratomas post-transplantation presents a formidable obstacle to their therapeutic utility. In previous studies, we identified an array of cell surface proteins specifically expressed in the pluripotent state, as revealed through proteomic analysis. Here we focused on EPHA2, a protein found to be abundantly present on the surface of undifferentiated mouse ESCs and is diminished upon differentiation. Knock-down of Epha2 led to the spontaneous differentiation of mouse ESCs, underscoring a pivotal role of EPHA2 in maintaining an undifferentiated cell state. Further investigations revealed a strong correlation between EPHA2 and OCT4 expression during the differentiation of both mouse and human PSCs. Notably, removing EPHA2+ cells from mouse ESC-derived hepatic lineage reduced tumor formation after transplanting them into immune-deficient mice. Similarly, in human iPSCs, a larger proportion of EPHA2+ cells correlated with higher OCT4 expression, reflecting the pattern observed in mouse ESCs. Conclusively, EPHA2 emerges as a potential marker for selecting undifferentiated stem cells, providing a valuable method to decrease tumorigenesis risks after stem-cell transplantation in regenerative treatments. | |||||||||||
| 書誌情報 |
en : Stem Cells Translational Medicine 巻 13, 号 8, p. 763-775, 発行日 2024-08-01 |
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| 出版者 | Oxford University Press | |||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||
| 収録物識別子 | 2157-6580 | |||||||||||
| 出版者版DOI | ||||||||||||
| 関連タイプ | isReplacedBy | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1093/stcltm/szae036 | |||||||||||
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| 関連タイプ | isReplacedBy | |||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://academic.oup.com/stcltm/article/13/8/763/7685021 | |||||||||||
| 権利 | ||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||||||||
| 権利情報 | $00A9 The Author(s) 2024. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||||
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| 出版タイプ | NA | |||||||||||
