| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-31 |
| タイトル |
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タイトル |
Ethylene glycol metabolism in the poly(ethylene terephthalate)-degrading bacterium Ideonella sakaiensis |
| 言語 |
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言語 |
eng |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Ideonella sakaiensis |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Poly(ethylene terephthalate) |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Ethylene glycol metabolism |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Alcohol dehydrogenase |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Aldehyde dehydrogenase |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Hachisuka, Shin-ichi
Chong, Jia Fong
Fujiwara, Tsuyoshi
Takayama, Akiyo
Kawakami, Yumiko
吉田, 昭介
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Poly(ethylene terephthalate) (PET)-degrading bacterium Ideonella sakaiensis produces hydrolytic enzymes that convert PET, via mono(2-hydroxyethyl) terephthalate (MHET), into the monomeric compounds, terephthalic acid (TPA), and ethylene glycol (EG). Understanding PET metabolism is critical if this bacterium is to be engineered for bioremediation and biorecycling. TPA uptake and catabolism in I. sakaiensis have previously been studied, but EG metabolism remains largely unexplored despite its importance. First, we identified two alcohol dehydrogenases (IsPedE and IsPedH) and one aldehyde dehydrogenase (IsPedI) in I. sakaiensis as the homologs of EG metabolic enzymes in Pseudomonas putida KT2440. IsPedE and IsPedH exhibited EG dehydrogenase activities with Ca2+ and a rare earth element (REE) Pr3+, respectively. We further found an upregulated dehydrogenase gene when the bacterium was grown on EG, whose gene product (IsXoxF) displays a minor EG dehydrogenase activity with Pr3+. IsPedE displayed a similar level of activity toward various alcohols. In contrast, IsPedH was more active toward small alcohols, whereas IsXoxF was the opposite. Structural analysis with homology models revealed that IsXoxF had a larger catalytic pocket than IsPedE and IsPedH, which could accommodate relatively bulkier substrates. Pr3+ regulated the protein expression of IsPedE negatively; IsPedH and IsXoxF were positively regulated. Taken together, these results indicated that the combination of IsPedH and IsXoxF complements the function of IsPedE in the presence of REEs. IsPedI exhibited dehydrogenase activity toward various aldehydes with the highest activity toward glycolaldehyde. This study demonstrated a unique alcohol oxidation pathway of I. sakaiensis, which could be efficient in EG utilization. |
| 書誌情報 |
en : Applied Microbiology and Biotechnology
巻 106,
号 23,
p. 7867-7878,
発行日 2022-10-27
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| 出版者 |
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出版者 |
Springer |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1432-0614 |
| 出版者版DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1007/s00253-022-12244-y |
| 出版者版URI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://link.springer.com/article/10.1007/s00253-022-12244-y |
| 権利 |
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|
権利情報 |
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00253-022-12244-y |
| 著者版フラグ |
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出版タイプ |
AM |
fulltext (3.9 MB)
