| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-10-30 |
| タイトル |
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タイトル |
Assaying Effector Cell-to-Cell Mobility in Plant Tissues Identifies Hypermobility and Indirect Manipulation of Plasmodesmata |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
大津, 美奈
Jennings, Joanna
Johnston, Matthew
Breakspear, Andrew
Liu, Xiaokun
Stark, Kara
Morris, Richard J.
de Keijzer, Jeroen
Faulkner, Christine
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In plants, plasmodesmata establish cytoplasmic continuity between cells to allow for communication and resource exchange across the cell wall. While plant pathogens use plasmodesmata as a pathway for both molecular and physical invasion, the benefits of molecular invasion (cell-to-cell movement of pathogen effectors) are poorly understood. To establish a methodology for identification and characterization of the cell-to-cell mobility of effectors, we performed a quantitative live imaging-based screen of candidate effectors of the fungal pathogen Colletotrichum higginsianum. We predicted C. higginsianum effectors by their expression profiles, the presence of a secretion signal, and their predicted and in planta localization when fused to green fluorescent protein. We assayed for cell-to-cell mobility of nucleocytosolic effectors and identified 14 that are cell-to-cell mobile. We identified that three of these effectors are “hypermobile,” showing cell-to-cell mobility greater than expected for a protein of that size. To explore the mechanism of hypermobility, we chose two hypermobile effectors and measured their impact on plasmodesmata function and found that even though they show no direct association with plasmodesmata, each increases the transport capacity of plasmodesmata. Thus, our methods for quantitative analysis of cell-to-cell mobility of candidate microbe-derived effectors, or any suite of host proteins, can identify cell-to-cell hypermobility and offer greater understanding of how proteins affect plasmodesmal function and intercellular connectivity. |
| 書誌情報 |
en : Molecular Plant-Microbe Interactions
巻 37,
号 2,
p. 84-92,
発行日 2024-02-26
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| 出版者 |
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出版者 |
American Phytopathological Society |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1943-7706 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1094/MPMI-05-23-0052-TA |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://apsjournals.apsnet.org/doi/full/10.1094/MPMI-05-23-0052-TA |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
Copyright $00A9 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| 著者版フラグ |
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出版タイプ |
NA |
