| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-10-25 |
| タイトル |
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タイトル |
In-Depth Quantification of Cell Division and Elongation Dynamics at the Tip of Growing Arabidopsis Roots Using 4D Microscopy, AI-Assisted Image Processing and Data Sonification |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Cell division |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Cell elongation |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Deep learning |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Root zonation |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
Sonification |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Time-lapse imaging |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
郷, 達明
Song, Yu
Yonekura, Takaaki
Obushi, Noriyasu
Den, Zeping
Imizu, Katsutoshi
Tomizawa, Yoko
Kondo, Yohei
Miyashima, Shunsuke
Iwamoto, Yutaro
Inami, Masahiko
Chen, Yen-Wei
中島, 敬二
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
One of the fundamental questions in plant developmental biology is how cell proliferation and cell expansion coordinately determine organ growth and morphology. An amenable system to address this question is the Arabidopsis root tip, where cell proliferation and elongation occur in spatially separated domains, and cell morphologies can easily be observed using a confocal microscope. While past studies revealed numerous elements of root growth regulation including gene regulatory networks, hormone transport and signaling, cell mechanics and environmental perception, how cells divide and elongate under possible constraints from cell lineages and neighboring cell files has not been analyzed quantitatively. This is mainly due to the technical difficulties in capturing cell division and elongation dynamics at the tip of growing roots, as well as an extremely labor-intensive task of tracing the lineages of frequently dividing cells. Here, we developed a motion-tracking confocal microscope and an Artificial Intelligence (AI)-assisted image-processing pipeline that enables semi-automated quantification of cell division and elongation dynamics at the tip of vertically growing Arabidopsis roots. We also implemented a data sonification tool that facilitates human recognition of cell division synchrony. Using these tools, we revealed previously unnoted lineage-constrained dynamics of cell division and elongation, and their contribution to the root zonation boundaries. |
| 書誌情報 |
en : Plant and Cell Physiology
巻 64,
号 11,
p. 1262-1278,
発行日 2023-10-17
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1471-9053 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/pcp/pcad105 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://academic.oup.com/pcp/article/64/11/1262/7323573 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc/4.0/ |
|
権利情報 |
$00A9 The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| 著者版フラグ |
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出版タイプ |
NA |
