| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-10-11 |
| タイトル |
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タイトル |
Arabidopsis BBX14 negatively regulates nitrogen starvation- and dark-induced leaf senescence |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
UDC |
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主題 |
Arabidopsis thaliana |
| キーワード |
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主題Scheme |
Other |
|
主題 |
BBX |
| キーワード |
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主題Scheme |
Other |
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主題 |
senescence |
| キーワード |
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主題Scheme |
Other |
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主題 |
transcription factor |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Buelbuel, Selin
Sakuraba, Yasuhito
Sedaghatmehr, Mastoureh
Watanabe, Mutsumi
Hoefgen, Rainer
Balazadeh, Salma
Mueller-Roeber, Bernd
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Senescence is a highly regulated process driven by developmental age and environmental factors. Although leaf senescence is accelerated by nitrogen (N) deficiency, the underlying physiological and molecular mechanisms are largely unknown. Here, we reveal that BBX14, a previously uncharacterized BBX-type transcription factor in Arabidopsis, is crucial for N starvation-induced leaf senescence. We find that inhibiting BBX14 by artificial miRNA (amiRNA) accelerates senescence during N starvation and in darkness, while BBX14 overexpression (BBX14-OX) delays it, identifying BBX14 as a negative regulator of N starvation- and dark-induced senescence. During N starvation, nitrate and amino acids like glutamic acid, glutamine, aspartic acid, and asparagine were highly retained in BBX14-OX leaves compared to the wild type. Transcriptome analysis showed a large number of senescence-associated genes (SAGs) to be differentially expressed between BBX14-OX and wild-type plants, including ETHYLENE INSENSITIVE3 (EIN3) which regulates N signaling and leaf senescence. Chromatin immunoprecipitation (ChIP) showed that BBX14 directly regulates EIN3 transcription. Furthermore, we revealed the upstream transcriptional cascade of BBX14. By yeast one-hybrid screen and ChIP, we found that MYB44, a stress-responsive MYB transcription factor, directly binds to the promoter of BBX14 and activates its expression. In addition, Phytochrome Interacting Factor 4 (PIF4) binds to the promoter of BBX14 to repress BBX14 transcription. Thus, BBX14 functions as a negative regulator of N starvation-induced senescence through EIN3 and is directly regulated by PIF4 and MYB44. |
| 書誌情報 |
en : The Plant Journal
巻 116,
号 1,
p. 251-268,
発行日 2023-06-29
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1365-313X |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/tpj.16374 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://onlinelibrary.wiley.com/doi/10.1111/tpj.16374 |
| 権利 |
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権利情報Resource |
http://creativecommons.org/licenses/by-nc/4.0/ |
|
権利情報 |
$00A9 2023 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| 著者版フラグ |
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出版タイプ |
NA |
