| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-09-06 |
| タイトル |
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タイトル |
Signal sequence-triage is activated by translocon obstruction sensed by an ER stress sensor IRE1α |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
endoplasmic reticulum |
| キーワード |
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主題Scheme |
Other |
|
主題 |
translocation capacity |
| キーワード |
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主題Scheme |
Other |
|
主題 |
translocon clogging |
| キーワード |
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主題Scheme |
Other |
|
主題 |
IRE1 |
| キーワード |
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主題Scheme |
Other |
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主題 |
signal sequence |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Sogawa,Ashuei
Komori,Ryota
Yanagitani, Kota
Ohfurudono, Miku
都留, 秋雄
Kadoi, Koji
木俣, 行雄
Yoshida, Hiderou
河野, 憲二
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Secretory pathway proteins are cotranslationally translocated into the endoplasmic reticulum (ER) of metazoan cells through the protein channel, translocon. Given that there are far fewer translocons than ribosomes in a cell, it is essential that secretory protein-translating ribosomes only occupy translocons transiently. Therefore, if translocons are obstructed by ribosomes stalled or slowed in translational elongation, it possibly results in deleterious consequences to cellular function. Hence, we investigated how translocon clogging by stalled ribosomes affects mammalian cells. First, we constructed ER-destined translational arrest proteins (ER-TAP) as an artificial protein that clogged the translocon in the ER membrane. Here, we show that the translocon clogging by ER-TAP expression activates triage of signal sequences (SS) in which secretory pathway proteins harboring highly efficient SS are preferentially translocated into the ER lumen. Interestingly, the translocon obstructed status specifically activates inositol requiring enzyme 1α (IRE1α) but not protein kinase R-like ER kinase (PERK). Given that the IRE1α$2013XBP1 pathway mainly induces the translocon components, our discovery implies that lowered availability of translocon activates IRE1α, which induces translocon itself. This results in rebalance between protein influx into the ER and the cellular translocation capacity. |
| 書誌情報 |
en : Cell Structure and Function
巻 48,
号 2,
p. 211-221,
発行日 2023-09-28
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| 出版者 |
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出版者 |
Japan Society for Cell Biology |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
0386-7196 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1247/csf.23072 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.jstage.jst.go.jp/article/csf/48/2/48_23072/_article |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/legalcode |
|
権利情報 |
$00A92023 The Author(s). This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited. |
| 著者版フラグ |
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出版タイプ |
NA |
