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Endoplasmic stress sensor Ire1 is involved in cytosolic/nuclear protein quality control in Pichia pastoris cells independent of HAC1
http://hdl.handle.net/10061/0002000532
http://hdl.handle.net/10061/0002000532c84ccc8c-a050-420a-93da-03e2483d30c0
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2024-09-06 | |||||||||
| タイトル | ||||||||||
| タイトル | Endoplasmic stress sensor Ire1 is involved in cytosolic/nuclear protein quality control in Pichia pastoris cells independent of HAC1 | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | unfolded protein response | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | yeast | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | Pichia pastoris | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | endoplasmic reticulum | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | stress response | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | journal article | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| 著者 |
Fauzee, Yasmin Nabilah Binti Mohd
× Fauzee, Yasmin Nabilah Binti Mohd
× Yoshida, Yuki
× 木俣, 行雄 |
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | In eukaryotic species, dysfunction of the endoplasmic reticulum (ER), namely, ER stress, provokes a cytoprotective transcription program called the unfolded protein response (UPR). The UPR is triggered by transmembrane ER-stress sensors, including Ire1, which acts as an endoribonuclease to splice and mature the mRNA encoding the transcription factor Hac1 in many fungal species. Through analyses of the methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii), we revealed a previously unknown function of Ire1. In P. pastoris cells, the IRE1 knockout mutation (ire1Δ) and HAC1 knockout mutation (hac1Δ) caused only partially overlapping gene expression changes. Protein aggregation and the heat shock response (HSR) were induced in ire1Δ cells but not in hac1Δ cells even under non-stress conditions. Moreover, Ire1 was further activated upon high-temperature culturing and conferred heat stress resistance to P. pastoris cells. Our findings cumulatively demonstrate an intriguing case in which the UPR machinery controls cytosolic protein folding status and the HSR, which is known to be activated upon the accumulation of unfolded proteins in the cytosol and/or nuclei. | |||||||||
| 書誌情報 |
en : Frontiers in Microbiology 巻 14, 発行日 2023-06-20 |
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| 出版者 | ||||||||||
| 出版者 | Frontiers Media | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 1664-302X | |||||||||
| 出版者版DOI | ||||||||||
| 関連タイプ | isReplacedBy | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.3389/fmicb.2023.1157146 | |||||||||
| 出版者版URI | ||||||||||
| 関連タイプ | isReplacedBy | |||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1157146/full | |||||||||
| 権利 | ||||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||
| 権利情報 | $00A9 2023 Fauzee, Yoshida and Kimata. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | NA | |||||||||
