| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-08-30 |
| タイトル |
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タイトル |
Multi-Region Microdialysis Imaging Platform Revealed Dorsal Raphe Nucleus Calcium Signaling and Serotonin Dynamics during Nociceptive Pain |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
calcium imaging |
| キーワード |
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主題Scheme |
Other |
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主題 |
DRN |
| キーワード |
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主題Scheme |
Other |
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主題 |
nociception |
| キーワード |
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主題Scheme |
Other |
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主題 |
pain |
| キーワード |
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主題Scheme |
Other |
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主題 |
serotonin |
| キーワード |
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主題Scheme |
Other |
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主題 |
micro-imaging device |
| キーワード |
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主題Scheme |
Other |
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主題 |
microdialysis |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Akbar, Latiful
Castillo, Virgil C.
Olorocisimo, Joshua P.
Ohta, Yasumi
Kawahara, Mamiko
Takehara, Hironari
春田, 牧人
田代, 洋行
笹川, 清隆
Ohsawa, Masahiro
Akay, Yasemin M.
Akay, Metin
太田, 淳
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
In this research, we combined our ultralight micro-imaging device for calcium imaging with microdialysis to simultaneously visualize neural activity in the dorsal raphe nucleus (DRN) and measure serotonin release in the central nucleus of the amygdala (CeA) and the anterior cingulate cortex (ACC). Using this platform, we observed brain activity following nociception induced by formalin injection in the mouse’s hind paw. Our device showed that DRN fluorescence intensity increased after formalin injection, and the increase was highly correlated with the elevation in serotonin release in both the CeA and ACC. The increase in calcium fluorescence intensity occurred during the acute and inflammatory phases, which suggests the biphasic response of nociceptive pain. Furthermore, we found that the increase in fluorescence intensity was positively correlated with mouse licking behavior. Lastly, we compared the laterality of pain stimulation and found that DRN fluorescence activity was higher for contralateral stimulation. Microdialysis showed that CeA serotonin concentration increased only after contralateral stimulation, while ACC serotonin release responded bilaterally. In conclusion, our study not only revealed the inter-regional serotonergic connection among the DRN, the CeA, and the ACC, but also demonstrated that our device is feasible for multi-site implantation in conjunction with a microdialysis system, allowing the simultaneous multi-modal observation of different regions in the brain. |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 24,
号 7,
発行日 2023-04-03
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| 出版者 |
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出版者 |
MDPI |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1422-0067 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/ijms24076654 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.mdpi.com/1422-0067/24/7/6654 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
$00A9 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
