| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-07-24 |
| タイトル |
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タイトル |
Arabidopsis thaliana Subclass I ACTIN DEPOLYMERIZING FACTORs Regulate Nuclear Organization and Gene Expression |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
Actin |
| キーワード |
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主題Scheme |
Other |
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主題 |
ACTIN DEPOLYMERIZING FACTOR |
| キーワード |
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主題Scheme |
Other |
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主題 |
Arabidopsis thaliana |
| キーワード |
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主題Scheme |
Other |
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主題 |
Heterochromatin |
| キーワード |
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主題Scheme |
Other |
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主題 |
Nucleus |
| キーワード |
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主題Scheme |
Other |
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主題 |
Regulation of gene expression |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Matsumoto, Tomoko
Higaki, Takumi
Takatsuka, Hirotomo
Kutsuna, Natsumaro
Ogata, Yoshiyuki
Hasezawa, Seiichiro
梅田, 正明
Inada, Noriko
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
ACTIN DEPOLYMERIZING FACTOR (ADF) is a conserved protein that regulates the organization and dynamics of actin microfilaments. Eleven ADFs in the Arabidopsis thaliana genome are grouped into four subclasses, and subclass I ADFs, ADF1$20134, are all expressed throughout the plant. Previously, we showed that subclass I ADFs function in the regulation of the response against powdery mildew fungus as well as in the regulation of cell size and endoreplication. Here, we report a new role of subclass I ADFs in the regulation of nuclear organization and gene expression. Through microscopic observation of epidermal cells in mature leaves, we found that the size of chromocenters in both adf4 and transgenic lines where expression of subclass I ADFs is downregulated (ADF1-4Ri) was reduced compared with that of wild-type Col-0. Arabidopsis thaliana possesses eight ACTIN (ACT) genes, among which ACT2, $22127 and $22128 are expressed in vegetative organs. The chromocenter size in act7, but not in the act2/8 double mutant, was enlarged compared with that in Col-0. Microarray analysis revealed that 1,818 genes were differentially expressed in adf4 and ADF1-4Ri. In particular, expression of 22 nucleotide-binding leucine-rich repeat genes, which are involved in effector-triggered plant immunity, was reduced in adf4 and ADF1-4Ri. qRT-PCR confirmed the altered expressions shown with microarray analysis. Overall, these results suggest that ADF regulates various aspects of plant physiology through its role in regulation of nuclear organization and gene expression. The mechanism how ADF and ACT regulate nuclear organization and gene expression is discussed. |
| 書誌情報 |
en : Plant and Cell Physiology
巻 64,
号 10,
p. 1231-1242,
発行日 2023-10-01
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1471-9053 |
| 出版者版DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/pcp/pcad092 |
| 出版者版URI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
URI |
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関連識別子 |
https://academic.oup.com/pcp/article/64/10/1231/7255892 |
| 権利 |
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権利情報 |
$00A9 The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record Tomoko Matsumoto, Takumi Higaki, Hirotomo Takatsuka, Natsumaro Kutsuna, Yoshiyuki Ogata, Seiichiro Hasezawa, Masaaki Umeda, Noriko Inada, Arabidopsis thaliana Subclass I ACTIN DEPOLYMERIZING FACTORs Regulate Nuclear Organization and Gene Expression, Plant and Cell Physiology is available online at: https://academic.oup.com/pcp/article/64/10/1231/7255892 and https://doi.org/10.1093/pcp/pcad092. 出版社許諾条件により、本文は2024年10月2日以降に公開 |
| 著者版フラグ |
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出版タイプ |
AM |
fulltext (6.5 MB)
