| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-06-14 |
| タイトル |
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タイトル |
Prominin-1 deletion results in spermatogenic impairment, sperm morphological defects, and infertility in mice |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
flagellum function |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Prom1 KO mice |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Prominin-1 |
| キーワード |
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主題Scheme |
Other |
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主題 |
sperm morphology |
| キーワード |
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主題Scheme |
Other |
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主題 |
spermatogenesis |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Matsukuma, Haruka
Kobayashi, Yuka
Oka, Shintaro
Higashijima, Fumiaki
Kimura, Kazuhiro
Yoshihara, Erika
笹井, 紀明
Shiraishi, Koji
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Purpose: Spermatogenesis is a complex process orchestrated by several essential genes. Prominin-1 (Prom1/PROM1) is a gene that is expressed in the testis but with a poorly understood role in spermatogenesis.
Methods: We used Prom1 knockout (Prom1 KO) mice to assess the role of Prom1 in spermatogenesis. To this end, we performed immunohistochemistry, immunofluorescence, western blotting, β-galactosidase staining, and apoptosis assay. Additionally, we analyzed the morphology of sperm and assessed litter sizes.
Results: We observed that PROM1 is localized to the dividing spermatocytes in seminiferous epithelial cells, sperm, and columnar epithelium in the epididymis. In the Prom1 KO testis, an aberrant increase in apoptotic cells and a decrease in proliferating seminiferous epithelial cells were observed. Cellular FLICE-like inhibitory protein (c-FLIP) and extracellular signal-regulated kinase 1/2 (ERK1/2) expression were also significantly decreased in Prom1 KO testis. In addition, a significantly increased number of epididymal spermatozoa with abnormal morphology and less motility was found in Prom1 KO mice.
Conclusions: PROM1 maintains spermatogenic cell proliferation and survival via c-FLIP expression in the testis. It is also involved in sperm motility and fertilization potential. The mechanism underlying the effect of Prom1 on sperm morphology and motility remains to be identified. |
| 書誌情報 |
en : Reproductive Medicine and Biology
巻 22,
号 1,
発行日 2023-06-06
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1447-0578 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/rmb2.12514 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://onlinelibrary.wiley.com/doi/10.1002/rmb2.12514 |
| 権利 |
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権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution inany medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. $00A9 2023 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. |
| 著者版フラグ |
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出版タイプ |
NA |
