| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-05-23 |
| タイトル |
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タイトル |
Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Bouslama, Rim
Dumont, Vincent
Lindfors, Sonja
Paavolainen, Lassi
Tienari, Jukka
Nisen, Harry
Mirtti, Tuomas
Saleem, Moin A.
Gordin, Daniel
Groop, Per-Henrik
末次, 志郎
Lehtonen, Sanna
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Changes in the dynamic architecture of podocytes, the glomerular epithelial cells, lead to kidney dysfunction. Previous studies on protein kinase C and casein kinase 2 substrates in neurons 2 (PACSIN2), a known regulator of endocytosis and cytoskeletal organization, reveal a connection between PACSIN2 and kidney pathogenesis. Here, we show that the phosphorylation of PACSIN2 at serine 313 (S313) is increased in the glomeruli of rats with diabetic kidney disease. We found that phosphorylation at S313 is associated with kidney dysfunction and increased free fatty acids rather than with high glucose and diabetes alone. Phosphorylation of PACSIN2 emerged as a dynamic process that fine-tunes cell morphology and cytoskeletal arrangement, in cooperation with the regulator of the actin cytoskeleton, Neural Wiskott$2013Aldrich syndrome protein (N-WASP). PACSIN2 phosphorylation decreased N-WASP degradation while N-WASP inhibition triggered PACSIN2 phosphorylation at S313. Functionally, pS313-PACSIN2 regulated actin cytoskeleton rearrangement depending on the type of cell injury and the signaling pathways involved. Collectively, this study indicates that N-WASP induces phosphorylation of PACSIN2 at S313, which serves as a mechanism whereby cells regulate active actin-related processes. The dynamic phosphorylation of S313 is needed to regulate cytoskeletal reorganization. |
| 書誌情報 |
en : Cells
巻 12,
号 11,
発行日 2023-05-27
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| 出版者 |
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出版者 |
MDPI |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2073-4409 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/cells12111487 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.mdpi.com/2073-4409/12/11/1487 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
$00A9 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
