| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-05-15 |
| タイトル |
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タイトル |
Dephosphorylation of neural wiring protein shootin1 by PP1 phosphatase regulates netrin-1-induced axon guidance |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
shootin1a |
| キーワード |
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主題Scheme |
Other |
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主題 |
PP1 |
| キーワード |
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主題Scheme |
Other |
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主題 |
Pak1 |
| キーワード |
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主題Scheme |
Other |
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主題 |
growth cone |
| キーワード |
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主題Scheme |
Other |
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主題 |
chemotaxis |
| キーワード |
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主題Scheme |
Other |
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主題 |
gradient sensing |
| キーワード |
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主題Scheme |
Other |
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主題 |
clutch molecule |
| キーワード |
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主題Scheme |
Other |
|
主題 |
desensitization |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Kastian, Ria Fajarwati
馬場, 健太郎
Kaewkascholkul, Napol
Sasaki, Hisashi
Watanabe, Rikiya
鳥山, 道則
稲垣, 直之
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Axon pathfinding is an essential step in neuronal network formation. Shootin1a is a clutch-linker molecule that is mechanically involved in axon outgrowth and guidance. It was previously shown that concentration gradients of axon guidance molecule netrin-1 in the extracellular environment elicit asymmetrically localized Pak1 kinase$2013mediated phosphorylation of shootin1a within axonal growth cones, which is higher on the netrin-1 source side. This asymmetric phosphorylation promotes shootin1a-mediated local actin$2013adhesion coupling within growth cones, thereby generating directional forces for turning the growth cone toward the netrin-1 source. However, how the spatial differences in netrin-1 concentration are transduced into the asymmetrically localized signaling within growth cones remains unclear. Moreover, the protein phosphatases that dephosphorylate shootin1a remain unidentified. Here, we report that protein phosphatase-1 (PP1) dephosphorylates shootin1a in growth cones. We found that PP1 overexpression abolished the netrin-1-induced asymmetric localization of phosphorylated shootin1a as well as axon turning. In addition, we show PP1 inhibition reversed the asymmetrically localized shootin1a phosphorylation within growth cones under netrin-1 gradient, thereby changing the netrin-1-induced growth cone turning from attraction to repulsion. These data indicate that PP1-mediated shootin1a dephosphorylation plays a key role in organizing asymmetrically localized phosphorylated shootin1a within growth cones, which regulates netrin-1-induced axon guidance. |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 299,
号 5,
発行日 2023-04-11
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1083-351X |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jbc.2023.104687 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://www.sciencedirect.com/science/article/pii/S0021925823003290 |
| 権利 |
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権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
$00A9 2023 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 著者版フラグ |
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出版タイプ |
NA |
