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Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking
http://hdl.handle.net/10061/0002000205
http://hdl.handle.net/10061/00020002055615402a-ef75-403b-9364-706bdf2a9fa9
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2024-04-18 | |||||||
| タイトル | ||||||||
| タイトル | Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ | research protocol | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| 著者 |
宮﨑, 亮次
× 宮﨑, 亮次× Akiyama, Yoshinori
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Information on protein-protein interactions is crucial in understanding protein-mediated cellular processes; however, analyzing transient and unstable interactions in living cells is challenging. Here, we present a protocol capturing the interaction between an assembly intermediate form of a bacterial outer membrane protein and β-barrel assembly machinery complex components. We describe steps for expression of a protein target, chemical crosslinking combined with in vivo photo-crosslinking and crosslinking detection procedures including immunoblotting. This protocol can be adapted to analyze interprotein interactions in other processes. For complete details on the use and execution of this protocol, please refer to Miyazaki et al. (2021). |
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| 書誌情報 |
en : STAR Protocols 巻 4, 号 2, 発行日 2023-03-17 |
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| 出版者 | ||||||||
| 出版者 | Cell Press | |||||||
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| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2666-1667 | |||||||
| 出版者版DOI | ||||||||
| 関連タイプ | isReplacedBy | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1016/j.xpro.2023.102178 | |||||||
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| 関連タイプ | isReplacedBy | |||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S2666166723001363?via%3Dihub | |||||||
| 権利 | ||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by-nc-nd/4.0 | |||||||
| 権利情報 | c 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | NA | |||||||
