| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-04-11 |
| タイトル |
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タイトル |
Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Mukherjee, Apratim
Ron, Jonathan Emanuel
Hu, Hooi Ting
西村, 珠子
Hanawa-Suetsugu, Kyoko
Behkam, Bahareh
Mimori-Kiyosue, Yuko
Gov, Nir Shachna
末次, 志郎
Nain, Amrinder Singh
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The cell migration cycle, well-established in 2D, proceeds with forming new protrusive structures at the cell membrane and subsequent redistribution of contractile machinery. Three-dimensional (3D) environments are complex and composed of 1D fibers, and 1D fibers are shown to recapitulate essential features of 3D migration. However, the establishment of protrusive activity at the cell membrane and contractility in 1D fibrous environments remains partially understood. Here the role of membrane curvature regulator IRSp53 is examined as a coupler between actin filaments and plasma membrane during cell migration on single, suspended 1D fibers. IRSp53 depletion reduced cell-length spanning actin stress fibers that originate from the cell periphery, protrusive activity, and contractility, leading to uncoupling of the nucleus from cellular movements. A theoretical model capable of predicting the observed transition of IRSp53-depleted cells from rapid stick-slip migration to smooth and slower migration due to reduced actin polymerization at the cell edges is developed, which is verified by direct measurements of retrograde actin flow using speckle microscopy. Overall, it is found that IRSp53 mediates actin recruitment at the cellular tips leading to the establishment of cell-length spanning fibers, thus demonstrating a unique role of IRSp53 in controlling cell migration in 3D. |
| 書誌情報 |
en : Advanced Science
巻 10,
号 7,
発行日 2023-01-25
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2198-3844 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/advs.202207368 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://onlinelibrary.wiley.com/doi/10.1002/advs.202207368 |
| 権利 |
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権利情報Resource |
http://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
$00A9 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.This is an open access article under the terms of the Creative CommonsAttribution License, which permits use, distribution and reproduction inany medium, provided the original work is properly cited. |
| 著者版フラグ |
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出版タイプ |
NA |
